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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 4049-4057
DOI: https://doi.org/10.20546/ijcmas.2018.708.420


Screening and Testing the Effect of Biocontrol Agents (Bacillus sp.) and Fusarium oxysporum f. sp. ciceri strains (Wilt) on Plant Defense Enzymes in Chickpea
Kiran K. Reddy* and K. Annapurna
Division of Microbiology, IARI, New Delhi-110012, India
*Corresponding author
Abstract:

Wilt (Fusarium oxysporum f. sp. ciceris) is considered as one of the major factors for low productivity of chickpea (Cicer arietinum L.). In order to address the problem, germinated seeds of ‘Desi’ chickpea cv. JG-62 were inoculated with a conidial suspension (root dip) of highly virulent Fusarium oxysporum f. sp. ciceris (Foc) race 4, three days after germination (DAG). The extent of disease suppression was studied with two bacterial biocontrol agents B-36 and MSUC-2. Out of seventeen bacterial isolated screened for antifungal activity, strain B-36 reported maximum inhibition zone of 15 mm with fungal strain 101, which has shown maximum virulence (55.6% reduction in plant stand). Chlorophyll and nitrogen content has reduced by 25.2% and 66% respectively at 30 DAS in pathogen inoculated treatment. β 1,3 glucanse, chitinase, phenylalanine ammonia lyase activities had 1.9, 2.02 and1.72fold increase respectively in B-36 treated seeds compared to pathogen only treated seeds. 16S rDNA sequencing of both the bacterial cultures identified them as Bacillus subtilis (Acc No: KX 503819) and Bacillus sp (Acc No: KX 503820) respectively


Keywords: Wilt, Days after germination (DAG), Chlorophyll
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How to cite this article:

Kiran K. Reddy and Annapurna, K. 2018. Screening and Testing the Effect of Biocontrol Agents (Bacillus sp.) and Fusarium oxysporum f. sp. ciceri strains (Wilt) on Plant Defense Enzymes in Chickpea.Int.J.Curr.Microbiol.App.Sci. 7(8): 4049-4057. doi: https://doi.org/10.20546/ijcmas.2018.708.420