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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 3965-3971
DOI: https://doi.org/10.20546/ijcmas.2018.708.409


Studies on Somatic Embryognesis in Chrysanthemum cv. Marigold Using Root and Leaf as Explants
Subhasini Ghosh1, Mahantesha B.N. Naika1*, Sandhyarani Nishani1, Mukund Shiragur2 and Amruta Bhat3
1Department of Biotechnology and crop improvement, K.R.C. College of Horticulture, Arabhavi-591218, University of Horticultural Sciences, Bagalkot, Karnataka, India
2Department of Floriculture and Landscape Architecture, K.R.C. College of Horticulture, Arabhavi-591218, University of Horticultural Sciences, Bagalkot, Karnataka, India
3Department of Horticulture Plant Pathology, K.R.C. College of Horticulture, Arabhavi-591218, University of Horticultural Sciences, Bagalkot, Karnataka, India
*Corresponding author
Abstract:

Chrysanthemum is a second most important flower crop globally. Marigold is a introduced cultivar gaining popularity among farmers and consumers of southern part of India due to its colour and high shelf life. Due to its commercial importance as quality planting material and cut flowers it is difficult to mitigate the gradually increasing demand by only vegetative means of propagation which is time consuming with low multiplication rate. Plant tissue culture is a better alternative tool to overcome conventional limitations. We have used leaf and root explants and cultured in MS medium containing different concentration of BAP, 2,4-D, NAA and different growth components such as L-proline and caesin hydrolysate. Root explants produced poor quality callus without any plant regeneration, whereas leaf explants showed somatic embryogenesis in turn plant regeneration in various degree in different media. From this study it is understood that the leaf explants can be successfully utilized for the plant regeneration through somatic embryogeneis in chrysanthemum.


Keywords: Chrysanthemum, Tissue culture, Root, Leaf, Somatic embryogenesis, Plant regeneration
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How to cite this article:

Subhasini Ghosh, Mahantesha B.N. Naika, Sandhyarani Nishani, Mukund Shiragur and Amruta Bhat. 2018. Studies on Somatic Embryognesis in Chrysanthemum cv. Marigold Using Root and Leaf as Explants.Int.J.Curr.Microbiol.App.Sci. 7(8): 3965-3971. doi: https://doi.org/10.20546/ijcmas.2018.708.409