National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Chrysanthemum is a second most important flower crop globally. Marigold is a introduced cultivar gaining popularity among farmers and consumers of southern part of India due to its colour and high shelf life. Due to its commercial importance as quality planting material and cut flowers it is difficult to mitigate the gradually increasing demand by only vegetative means of propagation which is time consuming with low multiplication rate. Plant tissue culture is a better alternative tool to overcome conventional limitations. We have used leaf and root explants and cultured in MS medium containing different concentration of BAP, 2,4-D, NAA and different growth components such as L-proline and caesin hydrolysate. Root explants produced poor quality callus without any plant regeneration, whereas leaf explants showed somatic embryogenesis in turn plant regeneration in various degree in different media. From this study it is understood that the leaf explants can be successfully utilized for the plant regeneration through somatic embryogeneis in chrysanthemum.
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