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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 3781-3786
DOI: https://doi.org/10.20546/ijcmas.2018.708.385


Protein Profile Analysis of Dermatophilus congolensis Isolates Using Sodium Dodecyl Polyacrylamide Gel Electrophoresis and Western Blotting
P.V. Tresamol, M.R. Saseendranath and S. Vamshi Krishna
Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciences, Mannuthy Kerala Veterinary and Animal Sciences University, India
*Corresponding author
Abstract:

Bovine dermatophilosis, also known as cutaneous streptothrichosis in cattle, is a skin infection which causes severe production losses in tropical countries. The present paper reports protein profile analysis of D. congolensis isolates from bovines in Kerala and identification of the immunodominant proteins using western blotting. Fifteen isolates of Dermatophilus congolensis obtained from dermatitis cases from 14 cattle and one buffalo were included in the study. Protein profile analysis of the isolates using sodium dodecyl polyacrylamide gel electrophoresis revealed variable number of bands with molecular weight in 14 to 130 kDa range. Quantitative as well as qualitative differences were observed in protein patterns obtained from different isolates which indicated the antigenic relatedness and diversity between the isolates. Western blotting of proteins of six isolates using hyper immune sera raised in rabbits recognised protein bands in the 25 to 35 KDa range. Proteins of molecular weights of about 29 KDa was found as major reactor in four of isolates tested, 32 KDa in two isolates, 38 KDa and 40 KDa in one each. The presence of the 29 KDa protein in majority of isolates in the present study indicates a possible role of it in inducing humeral immunity and therefore can be considered as a possible target for the immune response in cattle


Keywords: Dermatophilosis, Bovine, Protein profile, SDS-PAGE, Western blotting
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How to cite this article:

Tresamol, P.V., M.R. Saseendranath and Vamshi Krishna, S. 2018. Protein Profile Analysis of Dermatophilus congolensis Isolates Using Sodium Dodecyl Polyacrylamide Gel Electrophoresis and Western Blotting.Int.J.Curr.Microbiol.App.Sci. 7(8): 3781-3786. doi: https://doi.org/10.20546/ijcmas.2018.708.385