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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:8, August, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 3720-3728
DOI: https://doi.org/10.20546/ijcmas.2018.708.377


Mineral Profiling and Phytochemical Assessment of Lepidium sativum Seeds from Tropical Western India
Pushpendra Koli1*, Jitendra Kumar2,4, Braj B. Singh3 and Satyanshu. Kumar2
1ICAR-Indian Grassland and Fodder Research Institute, Jhansi-284003, UP
2ICAR-Directorate of Medicinal and Aromatic Plants Research, Anand-387310, Gujarat
3ICAR-Indian Agricultural Research Institute, New Delhi-110012
4Institute of Pesticide Formulation Technology, Gurugram, Haryana
*Corresponding author
Abstract:

From the time unknown, Lepidium sativum, an edible herb, has been recognized as an important medicinal plant. It has also been known to show good galactogogue property. In the present study, mineral profiling and phytochemical analysis of Lepidium seeds have been presented with the aim to recognize its importance as livestock feed. Mineral profiling was carried out with Flame Photometer and Atomic Absorption Spectrophotometer (AAS) and results showed the occurrence of K (11.9 g/kg), Na (1.23 g/kg), Ca (2.91 g/kg), Mg (3.15 g/kg), Zn (0.045 g/kg), Cu (0.006 g/kg), Fe (0.072 g/kg) and Mn (0.031 g/kg). For phytochemical assessment, oil was extracted from the crushed seed powder using soxhlet extraction method and 25.36 % yield was recorded. Further, the phenolic extraction through refluxing of defatted seed powder was done to determine other biochemical contents and yield of phenolic extract was obtained around 20.23 %. Total phenolic content determined through Folin test was 8.53 mg/g of seeds. Crude fibers in seed meal were also estimated to be 23 % in yield. Glucosinolate content was found 89 µmoles/g of seeds. Total flavonoids content was found to be 3.89 mg/g of seeds in phenolic extracts. DPPH (2,2- Diphenyl-1-picryl-hydrazine) free radical scavenging method was used to measure antioxidant activity and IC50 was found to be 21 ppm for methanolic extract. The present studies have revealed that L. sativum seeds have important mineral nutrients, fibers and other essential biochemical constituents and hence considered useful as animal feed and its health.


Keywords: Lepidium sativum, Livestock feed, Glucosinolate, DPPH, AAS, Mineral profiling

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How to cite this article:

Pushpendra Koli, Jitendra Kumar, Braj B. Singh and Satyanshu. Kumar. 2018. Mineral Profiling and Phytochemical Assessment of Lepidium sativum Seeds from Tropical Western India.Int.J.Curr.Microbiol.App.Sci. 7(8): 3720-3728. doi: https://doi.org/10.20546/ijcmas.2018.708.377
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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