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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 3682-3688
DOI: https://doi.org/10.20546/ijcmas.2018.708.373


Molecular Characterisation of Clostridium perfringens Type D Isolated from Sheep in Kashmir Himalayas, India
Z.A. Kashoo, S.A. Wani, A.H. Wani, S.M. Khan, S. Qureshi, I. Hussain, S. Farooq and J.A. Malla
Niche Area of Excellence, Anaerobic Bacteriology laboratory, Division of Veterinary Microbiology and Immunology, Faculty of Veterinary Sciences and Animal Husbandry, SKUAST-Kashmir, Shuhama, Srinagar-190006, India
*Corresponding author
Abstract:

The molecular characterization of Clostridium perfringens in sheep for various toxinotypes was investigated in this study. A total of 147 samples collected from healthy, diarrhoeic animals and morbid material of animals suspected to have died of enterotoxaemia were screened for Clostridium perfringens (C. perfringens) toxinotypes. The polymerase chain reaction (PCR) amplification of 16S rRNA gene revealed that out of 147 samples collected, 92 (62.58%) were found positive for C. perfringens. All the 92 isolates were screened for three toxin genes viz., cpa, cpb and etx using a multiplex PCR. Toxinotyping revealed that 65 (72.65%) were positive for Clostridium perfringens Type A and 27 (29.34%) were that of Clostridium perfringens Type D None of the isolates was found to be toxinotype B or C.


Keywords: Clostridium perfringens, Enterotoxaemia, Toxinotype, 16S rRNA, Multiplex PCR, cpa, cpb, etx.
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How to cite this article:

Kashoo, Z.A., S.A. Wani, A.H. Wani, S.M. Khan, S. Qureshi, I. Hussain, S. Farooq and Malla, J.A. 2018. Molecular Characterisation of Clostridium perfringens Type D Isolated from Sheep in Kashmir Himalayas, India.Int.J.Curr.Microbiol.App.Sci. 7(8): 3682-3688. doi: https://doi.org/10.20546/ijcmas.2018.708.373