Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 3599-3611
DOI: https://doi.org/10.20546/ijcmas.2018.708.364


Actinomycetes from the Coffee Plantation Soils of Western Ghats: Diversity and Enzymatic Potentials
Banu Sameera1, Harishchandra Sripathy Prakash2 and Monnanda Somaiah Nalini1*
1Department of Studies in Botany
2Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore–570 006, Karnataka, India
*Corresponding author
Abstract:

230 soil actinomycetes were isolated from the coffee plantation of Western Ghats, Karnataka, India along the altitudinal gradients and depths. 24 morphologically distinct species were obtained based on the aerial spore chains and by the sequencing of the 16S rRNA gene. The strains were assigned to the order Micrococcales, and novel orders Pseudonocardiales ord. nov., Streptomycetales ord. nov., and Streptosporangiales ord. nov. The frequently isolated genus was Streptomyces, along with rare actinomycetes Actinomadura, Spirillospora, Actinocorallia, Arthrobacter, Saccharopolyspora and Nonomuraea. This study is the first report on Nonomuraea antimicrobica as a soil actinomycete. Diversity studies on the distribution of soil actinomycetes indicated significant differences (P< 0.05) among Shannon diversity indices of sample group depths along the slope. An attempt was made to correlate the total actinomycete count with soil parameters, by PCA based multiple linear regression (MLR) which significantly correlated (P<0.0001) with pH, moisture, available nitrogen and phosphorous. About 91.6% of the isolates screened were found to be potentials for enzymatic activity. The most active enzyme producer Streptomyces sp. MH470335 produced 18.51, 1.53, 6.92, 5.62 and 5.15 U/ml for cellulase, pectinase, xylanase, amylase and protease respectively. Plantation soil actinomycetes showing enzymatic activities in vitro may indicate the potential for their use as stabilized biocatalysts.


Keywords: Plantation soils, Coffea arabica, Streptomycetes, Rare actinomycetes, Soil properties, enzymes
Download this article as Download

How to cite this article:

Banu Sameera, Harishchandra Sripathy Prakash and Monnanda Somaiah Nalini. 2018. Actinomycetes from the Coffee Plantation Soils of Western Ghats: Diversity and Enzymatic Potentials.Int.J.Curr.Microbiol.App.Sci. 7(8): 3599-3611. doi: https://doi.org/10.20546/ijcmas.2018.708.364