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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 3251-3264
DOI: https://doi.org/10.20546/ijcmas.2018.708.347


Studies of Ploidy Assessment in Some Synthetic Hybrids of Banana (Musa spp.)
Sukhen Chandra Das1, T.N. Balamohan2, K. Poornima3 and I. Van den Bergh4
1Department of Horticulture, College of Agriculture, Tripura, India
2Department of Fruit Crop, HC and RI, TNAU, Tamil Nadu, India
3Department of Nematology, HC and RI, TNAU, Tamil Nadu, India
4International Network for the Improvement of Banana and Plantain-Asia and the Pacific, c/o IRRI Khush Hall, Los Banos, Laguna 4031, Philippines
*Corresponding author
Abstract:

Banana (Musa spp.) constitute a hybrid-polyploid complex and are classified according to different genome compositions such as AA, BB, AB, AAA, AAB, ABB, AAAA, ABBB, AAAB, AABB and AAABB. Knowledge of ploidy and exact genome compositions of the parental material is essential for Musa breeding. Flow cytometric analysis of nuclear DNA content was used to estimate ploidy levels. Twenty four Banana hybrids under phase-I and nineteen hybrids under phase-II evaluated and was done by flow cytometry analysis which enables rapid and precise measurements on whole cells, isolated nuclei or chromosomes in a monodisperse suspension. Studies found under phase-I that six hybrids diploids (AA and AB), five hybrids triploids (AAA and AAB), ten hybrids tetraploids (AABB) and three hybrids pentaploids (AAABB) were recorded and under phase-II found that one hybrid diploids(AB), three triploids (AAB) and rest of the hybrids tetraploids (AABB) were recorded.


Keywords: Banana, Hybrids, Ploidy assessment
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How to cite this article:

Sukhen Chandra Das, T.N. Balamohan, K. Poornima and Van den Bergh, I. 2018. Studies of Ploidy Assessment in Some Synthetic Hybrids of Banana (Musa spp.).Int.J.Curr.Microbiol.App.Sci. 7(8): 3251-3264. doi: https://doi.org/10.20546/ijcmas.2018.708.347