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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:8, August, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 3041-3052
DOI: https://doi.org/10.20546/ijcmas.2018.708.324


Enumeration, Isolation and Identification of Diazotrophic Bacteria in Rhizosphere Soil of Two Rice Varieties in Jammu District
Harkirat Singh and Renu Gupta*
Division of Soil Science and Agricultural Chemistry, Faculty of Agriculture, SKUAST-J, Chatha, Jammu, India
*Corresponding author
Abstract:

Free living diazotrophic bacteria bacteria were isolated from the rhizosphere soil of two cultivated rice varieties grown in R. S. Pura and Bishnah blocks of Jammu district of Jammu Division of J&K State. Soil samples were collected from rice rhizosphere at panicle initiation stage. A total of twenty rice grown villages were selected and from each village two composite samples were taken from five plants one each from variety Basmati-370 and Ratna through random sampling from each rice growing village of R. S. Pura and Bishnah blocks of Jammu district by using GPS. The bacterial isolates characterized by various biochemical tests performed on them were identified by characteristics as outlined in Bergeys Manual of Systematic Bacteriology. The physico chemical properties of the soil were also estimated and correlated with changes in different environmental factors such as soil moisture, pH, EC, OC, N, P, K contents in different areas of R. S. Pura and Bishnah blocks and found to have influenced nitrogen fixation in soil thus affecting nitrogen fixers in the soil. The study highlighted the aerobic and anaerobic nature of isolates based on biochemical tests viz. catalase, nitrate reduction, citrate utilization and oxidase tests performed on them.


Keywords: Enumeration, Nitrogen fixers, Biochemical tests, Azotobacter, Beijerinckia, Characterization, Identification

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How to cite this article:

Harkirat Singh and Renu Gupta. 2018. Enumeration, Isolation and Identification of Diazotrophic Bacteria in Rhizosphere Soil of Two Rice Varieties in Jammu District.Int.J.Curr.Microbiol.App.Sci. 7(8): 3041-3052. doi: https://doi.org/10.20546/ijcmas.2018.708.324
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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