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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 3041-3052
DOI: https://doi.org/10.20546/ijcmas.2018.708.324


Enumeration, Isolation and Identification of Diazotrophic Bacteria in Rhizosphere Soil of Two Rice Varieties in Jammu District
Harkirat Singh and Renu Gupta*
Division of Soil Science and Agricultural Chemistry, Faculty of Agriculture, SKUAST-J, Chatha, Jammu, India
*Corresponding author
Abstract:

Free living diazotrophic bacteria bacteria were isolated from the rhizosphere soil of two cultivated rice varieties grown in R. S. Pura and Bishnah blocks of Jammu district of Jammu Division of J&K State. Soil samples were collected from rice rhizosphere at panicle initiation stage. A total of twenty rice grown villages were selected and from each village two composite samples were taken from five plants one each from variety Basmati-370 and Ratna through random sampling from each rice growing village of R. S. Pura and Bishnah blocks of Jammu district by using GPS. The bacterial isolates characterized by various biochemical tests performed on them were identified by characteristics as outlined in Bergeys Manual of Systematic Bacteriology. The physico chemical properties of the soil were also estimated and correlated with changes in different environmental factors such as soil moisture, pH, EC, OC, N, P, K contents in different areas of R. S. Pura and Bishnah blocks and found to have influenced nitrogen fixation in soil thus affecting nitrogen fixers in the soil. The study highlighted the aerobic and anaerobic nature of isolates based on biochemical tests viz. catalase, nitrate reduction, citrate utilization and oxidase tests performed on them.


Keywords: Enumeration, Nitrogen fixers, Biochemical tests, Azotobacter, Beijerinckia, Characterization, Identification
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How to cite this article:

Harkirat Singh and Renu Gupta. 2018. Enumeration, Isolation and Identification of Diazotrophic Bacteria in Rhizosphere Soil of Two Rice Varieties in Jammu District.Int.J.Curr.Microbiol.App.Sci. 7(8): 3041-3052. doi: https://doi.org/10.20546/ijcmas.2018.708.324