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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Fowl adenoviruses (FAdVs) are economically significant pathogens of domestic poultry. Fowl adenoviruses are associated with many disease conditions in domestic fowl such as IBH, IBH-HPS, Egg drop syndrome, Quail bronchitis, and gizzard erosions etc. The present study deals with epidemiological investigation of forty natural outbreaks of IBH/IBH-HPS in broiler farms of Uttar Pradesh and Uttarakhand during 2011-2016. Involvement of fowl adenovirus was suspected on the basis of post mortem lesions, which was confirmed by AGID, histopathology, virus isolation and hexon gene (L1 loop) PCR. Disease was mainly present in 3 to 6 week age broiler birds; however, birds less than 3 weeks of age were also affected. Mortality was in the range of 0.5% to 20% and disease incidence was found to be more in August and September months of the year. In AGID with known antiserum, viral antigen was indicated by presence of single precipitin line for each isolate. Histopathology of liver revealed presence of basophilic intra nuclear inclusion bodies. Virus isolates were successfully propagated in CEL cell culture and a band of ~900bp was observed in PCR. In histology, intra nuclear inclusion body was found in hepatocyte.