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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 2442-2459
DOI: https://doi.org/10.20546/ijcmas.2018.708.247


An Efficient Method for Qualitative Screening of Ligninolytic Enzyme Potential of Ganoderma lucidum
Harsimran Kaur, S. Kapoor and Shivani Sharma*
Department of Microbiology, Punjab Agricultural University, Ludhiana-141004, Punjab, India
*Corresponding author
Abstract:

This research aimed to evaluate the potential screening of different strains of white rot fungi Ganoderma lucidum to produce different ligninolytic enzymes which play an important role in transformation and mineralization of various organic pollutants, production of commercially valuable biobased products delignify paper, animal feed and biofuel production. The qualitative screening is a crucial step in isolation and selection of potential lignolytic fungi. Four strains of Ganoderma lucidum, a well-known medicinal fungus, were used to quantify the qualitative screening process for their ligninolytic activities by using different concentrations of three chromogenic indicators viz., ABTS, guaiacol and RBBR. Among the three tested indicators, 0.08 % ABTS concentration gave better visual expressions for ligninolytic activities of the fungal strains, followed by 0.075 % guaiacol. GL-1 and GL-2 strains were found to be hyper-ligninolytic strains with higher potency index values and higher tolerance capabilities towards the chromogenic indicator compounds; whereas GL-3 and GL-4 strains were grouped as hypo-ligninolytic strains due to their low potency index values. These strains with higher ligninolytic activities can be considered as potential candidates for development of an efficient biological system for the treatment of textile industry wastewaters and bioremediation of polluted ecosystems.


Keywords: ABTS, Ganoderma lucidum, Guaiacol, Ligninolytic, RBBR
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How to cite this article:

Harsimran Kaur, S. Kapoor and Shivani Sharma. 2018. An Efficient Method for Qualitative Screening of Ligninolytic Enzyme Potential of Ganoderma lucidum.Int.J.Curr.Microbiol.App.Sci. 7(8): 2442-2459. doi: https://doi.org/10.20546/ijcmas.2018.708.247