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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 2375-2384
DOI: https://doi.org/10.20546/ijcmas.2018.708.239


Molecular Diversity among the Isolates of Xanthomonas axonopodis pv. citri Causing Bacterial Canker in Citrus
Rutuja R. Kharde1, Shivaji A. Lavale2 and Bhausaheb Baban Ghorpade1*
1College of Agricultural Biotechnology, Loni, Ahmednagar, MS, India
2College of Agricultural Biotechnology, Takli-Kazi, Ahmednagar, MS, India
*Corresponding author
Abstract:

Genetic variability study of five isolates of Xanthomonas axonopodis pv. citri was carried out using molecular markers. The isolates were collected from five places in Maharashtra state, India. Morphological and Biochemical tests viz. gram’s reaction, starch hydrolysis, indole production, catalase test, KOH test, Gelatin liquefaction and Acid production test were carried out to identify and confirm the purity of the culture. The genetic variability was studied by using 14 RAPD and 8 ISSR primers out of which 7 RAPD primers produced 47 scorable bands and 2 ISSR primers produced 13 scorable bands. The similarity coefficient value ranged from 0.13 to 0.40 for RAPD and from 0.00 to 1.00 for ISSR primers across five isolates indicating high degree of genetic variation.


Keywords: Molecular diversity, RAPD, ISSR, Cluster analysis, Xanthomonas axonopodis pv. citri
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How to cite this article:

Rutuja R. Kharde, Shivaji A. Lavale and Bhausaheb Baban Ghorpade. 2018. Molecular Diversity among the Isolates of Xanthomonas axonopodis pv. citri Causing Bacterial Canker in Citrus.Int.J.Curr.Microbiol.App.Sci. 7(8): 2375-2384. doi: https://doi.org/10.20546/ijcmas.2018.708.239