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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 2360-2363
DOI: https://doi.org/10.20546/ijcmas.2018.708.237


Pathological and Molecular Diagnosis of Paratuberculosis in Sheep and Goats
P. Kanimozhi, M. Geetha*, K.M. Palanivel and M. Arthanari Eswaran
Department of Veterinary Preventive Medicine, Veterinary College and Research Institute, Namakkal, Tamil Nadu Veterinary and Animal Sciences University, Tamil Nadu, India
*Corresponding author
Abstract:

Johne’s disease (JD) is caused by Mycobacterium avium Subsp. Paratuberculosis (MAP) and the disease is characterized by chronic diarrhea, progressive weight loss and emaciation. The present study was carried out to find out the MAP infection status by postmortem and molecular diagnosis in sheep and goats suspected for JD. Postmortem examination of six small ruminants (One sheep and five goats) revealed the pathognomonic necrotic findings such as characterised hypertrophy of mucosal surfaces of small intestine (terminal ileum) and cord like thickening of mesenteric lymphnodes. Acid fast staining of cytological smears prepared from the intestinal scrapings and mesenteric lymphnode of all animals revealed presence of clusters of acid fast bacilli. Mesentric lymphnode and intestine were collected from during postmortem examination and subjected to polymerase chain reaction (PCR) using specific primers targeting IS 900 gene sequence of MAP which revealed positivity to MAP infection. The study concluded that postmortem examination combined with polymerase chain reaction is highly useful in accurate diagnosis of JD in small ruminants.


Keywords: Johne's disease, Mycobacterium Avium Subsp. Paratuberculosis, PCR, Sheep and goats
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How to cite this article:

Kanimozhi, P., M. Geetha, K.M. Palanivel and Arthanari Eswaran, M. 2018. Pathological and Molecular Diagnosis of Paratuberculosis in Sheep and Goats.Int.J.Curr.Microbiol.App.Sci. 7(8): 2360-2363. doi: https://doi.org/10.20546/ijcmas.2018.708.237