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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(9): 837-842
DOI: http://dx.doi.org/10.20546/ijcmas.2016.509.094


Genetic Diversity and Relationship of Selected Cultivars of Rice, Oryza sativa L. using Random Amplified Polymorphic DNA (RAPD) Markers
B.R. Roopa and B.K. Chikkaswamy*
Department of Biotechnology, Sigma Bioscience Research Center, # 530, 16th Cross, 2nd Stage, Indira Nagar, Bangaluru-560038, India
*Corresponding author
Abstract:

The genetic diversity in 26 selected cultivars of rice, Oryza sativa L. using 20 decamer random primers. Out of 20, 15 RAPD primers revealed polymorphism while the remaining 5 primers showed no reaction. The primers produced a total of 71 bands of which 66 were polymorphic (95.2%). The number of polymorphic fragments for each primer varied from 1to3 with an average of 2.53 polymorphic fragments. The primer OPA-19 produced the maximum number of polymorphic bands. The RAPD data was analyzed to determine the genetic similarity coefficients which ranged from 0.46 to 0.81. Cluster analysis was performed using Ward’s method) using the Jaccard’s similarity coefficient. The Wards method dendrogram resolved the selected rice cultivars into two to three  major clusters.


Keywords: Genetic variation molecular markers, Oryza sativa, Ward’s method
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How to cite this article:

Roopa, B.R.,  and Chikkaswamy, B.K. 2016. Genetic Diversity and Relationship of Selected Cultivars of Rice, Oryza sativa L. using Random Amplified Polymorphic DNA (RAPD) Markers.Int.J.Curr.Microbiol.App.Sci. 5(9): 837-842. doi: http://dx.doi.org/10.20546/ijcmas.2016.509.094