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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 1672-1682
DOI: https://doi.org/10.20546/ijcmas.2018.708.192


Exploration of Trichoderma spp. as an Effective Bio Control Agents against the Sclerotial wilt Caused by Sclerotium rolfsii Sacc.
M. Priyadharcini1, R. Akila1*, M.L. Mini2, N. Rajinimala1 and R. Kannan1
1Department of Plant Pathology
2Department Biochemistry, Soil Science and Agricultural Chemistry, Tamil Nadu Agricultural University, AC&RI, Killikulam, Vallanad-628 252, Tuticorin District, Tamil Nadu, India
*Corresponding author
Abstract:

Jasmine is the most attractive flower crop being cultivated throughout the tropical and sub-tropical countries. In Tamil Nadu jasmine cultivation is highly interrupted by the soil borne wilt pathogen Sclerotium rolfsii Sacc. In the current scenario of disease management of soil borne pathogens Trichoderma spp can be effectively exploited for the effective containment of the disease. The isolate SR1 was found to be the most virulent during the surveillance programme and it produced the maximum amount of host non-specific toxin oxalic acid. The Trichoderma species such as T. harzianum (TspT), Trichoderma sp. (TspK) and T. viride 1 performed well and inhibited the mycelial growth effectively. The best isolate TspT excelled in toxin degradation. The ITS region of TspT was amplified with primers ITS1 and ITS4 and the product (650 bp) showed homology sequences (98%) with T. harzianum in the NCBI-Blast search.


Keywords: Sclerotium rolfsii, Jasmine, ITS region, Oxalic acid, Volatile metabolites, Trichoderma harzianum
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How to cite this article:

Priyadharcini, M., R. Akila, M.L. Mini, N. Rajinimala and Kannan, R. 2018. Exploration of Trichoderma spp. as an Effective Bio Control Agents against the Sclerotial wilt Caused by Sclerotium rolfsii Sacc.Int.J.Curr.Microbiol.App.Sci. 7(8): 1672-1682. doi: https://doi.org/10.20546/ijcmas.2018.708.192