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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 1616-1627
DOI: https://doi.org/10.20546/ijcmas.2018.708.185


Bacterial Cellulose Production from Mature Black Spear Grass Hydrolysate by Gluconacetobacter xylinus: Effect of pH, Fermentation Time and Nitrogen Supplementation
C.G. Dirisu1,2 and W. Braide2
1Department of Biology, Federal College of Education (Technical) Omoku, Nigeria
2Department of Microbiology, Federal University of Technology Owerri, Nigeria
*Corresponding author
Abstract:

Mature black spear grass (Heteropogon contortus) hydrolysate (SGH) was used for the first time as lignocellulosic substrate for production of bacterial nanocellulose (BC) by Gluconacetobacter xylinum at 30oC and pH 5.5. The maximum total reducing sugar (TRS) from SGH was 2.48g/L using 6% H2SO4 at 121oC for 4h with higher amount of xylose than glucose and arabinose. BC yield was comparable to those from reported lignocellulosic hydrolysates. At the end of 15 days, mean BC yield (g/L) was 3.84+2.23. pH produced a significant effect on BC yield (p<0.05). There was a steady increase from an initial pH4 which later declined with further increase in pH. The maximum BC yield (g/L) (4.88+1.25) occurred at pH 6. The optimal pH was 5-6. Significant effect of fermentation time (FT) and BC on one hand and pH on BC on the other hand was determined statistically using regression model. Supplementation of SGH with nitrogen sources- yeast extract (YE), peptone or both did not increase BC yield above the value observed for SGH. There was however significant difference in BC yield from basal medium supplemented with the nitrogen sources, F (3, 4) =772, p<0.05.


Keywords: Bacterial nanocellulose, Spear grass hydrolysate, pH, Gluconacetobacter xylinus
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How to cite this article:

Dirisu, C.G. and Braide, W. 2018. Bacterial Cellulose Production from Mature Black Spear Grass Hydrolysate by Gluconacetobacter xylinus: Effect of pH, Fermentation Time and Nitrogen Supplementation.Int.J.Curr.Microbiol.App.Sci. 7(8): 1616-1627. doi: https://doi.org/10.20546/ijcmas.2018.708.185