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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 1540-1547
DOI: https://doi.org/10.20546/ijcmas.2018.708.176


Expression and Evaluation of Immuno-Reactivity of Recombinant VP2 Protein of Bluetongue Virus Serotype-23
Priya Sharma, Karam Chand*, Sanchay Kumar Biswas, M. Sankar, Arpit Saxena, Sujoy Tamang, Ankita Lohumi and Muthannan Andavar Ramakrishnan
Division of Virology, ICAR-Indian Veterinary Research Institute, Mukteswar Campus, Dist. Nainital 263 138, Uttarakhand, India
*Corresponding author
Abstract:

Bluetongue is an arthropod-borne, non-contagious viral disease of domestic and wild ruminants. In the present study, truncated VP2 protein of BTV serotype 23 (BTV-23) was expressed in a prokaryotic expression system and assessed for its potential use as a candidate for the development of serotype-specific BTV diagnostics assay. The expression of the VP2 protein was carried out in E. coli BL21 Codon Plus (DE3) cells using the pET32a expression vector. The purity of recombinant protein VP2 (rVP2) was analyzed in SDS-PAGE and Western blotting. To evaluate the immune reactivity and cross-reactivity of rVP2, homologous and heterologous BTV hyperimmune serum (HIS) were used respectively. There was no cross-reactivity of rVP2 BTV-23 protein with HIS against BTV-1. The expressed rVP2 protein may be used as a diagnostic antigen for the development of serotype-specific ELISA to detect antibody against BTV-23 serotypes in serum samples.


Keywords: Bluetongue virus, BTV-23, Recombinant VP2 protein, Immuno-reactivity
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How to cite this article:

Priya Sharma, Karam Chand, Sanchay Kumar Biswas, M. Sankar, Arpit Saxena, Sujoy Tamang, Ankita Lohumi and Muthannan Andavar Ramakrishnan. 2018. Expression and Evaluation of Immuno-Reactivity of Recombinant VP2 Protein of Bluetongue Virus Serotype-23.Int.J.Curr.Microbiol.App.Sci. 7(8): 1540-1547. doi: https://doi.org/10.20546/ijcmas.2018.708.176