National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Bluetongue is an arthropod-borne, non-contagious viral disease of domestic and wild ruminants. In the present study, truncated VP2 protein of BTV serotype 23 (BTV-23) was expressed in a prokaryotic expression system and assessed for its potential use as a candidate for the development of serotype-specific BTV diagnostics assay. The expression of the VP2 protein was carried out in E. coli BL21 Codon Plus (DE3) cells using the pET32a expression vector. The purity of recombinant protein VP2 (rVP2) was analyzed in SDS-PAGE and Western blotting. To evaluate the immune reactivity and cross-reactivity of rVP2, homologous and heterologous BTV hyperimmune serum (HIS) were used respectively. There was no cross-reactivity of rVP2 BTV-23 protein with HIS against BTV-1. The expressed rVP2 protein may be used as a diagnostic antigen for the development of serotype-specific ELISA to detect antibody against BTV-23 serotypes in serum samples.
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