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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 1187-1194
DOI: https://doi.org/10.20546/ijcmas.2018.708.133


Antimicrobial Activity of the Crude Extract Derived from Streptomyces spp. Associated with Sponges
S. Parimala1, M. Kandhasamy*, and P.S. Chinnasamy1
PG and Research Department of Botany, Government Arts College (Autonomous), Karur – 639 005, India
*Corresponding author
Abstract:

The objective of the present investigation was to isolate and identify the antimicrobial activity showing actinomycetes associated with sponges. Sponges such as Acanthella klethra, Theonella cylendrica, Helicona sp., Auletta sp., and Callyspongia sp. were collected at Sangumal, in Rameswaram island. Ramanathapuram District, Tamil Nadu, India. Isolates of 22 actinomycetes were isolated from sponges. Isolates No. 16 showed high antibacterial and antifungal activity and the isolate was identified as Streptomyces sp. Antibacterial and antifungal activity was determined by agar diffusion method and Minimum Inhibitory Concentration (MIC) of the extract was determined by two fold dilution method using microstate plate. The study showed that fungal pathogens were more susceptible to the extract than bacteria. Among bacterial pathogens, Escherichia coli was more susceptible to the extract, followed by P. aeruginosa, Bacillus subtilis, and Staphylococcus aureus and their growth were inhibited at the concentration of 2 mg/ml. Other bacterial pathogens were resistant to the crude extract derived from culture broth extract of Streptomyces sp. Fungal pathogens such as Candida albicans, Zynomonas sp., Aspergillus niger and A. rutus were susceptible to the extract and the MIC for the fungal pathogens ranged between 0.015 mg/ml and 0.025 mg/ml. A. rutus was susceptible to the extract and it growth was inhibited at the concentration less than 0.1µg/ml. Since the inhibitory concentrations against the fungal pathogens were too less, its purified form may be the potential source for controlling fungal pathogens.


Keywords: Sponges, Actinomycetes, Human pathogens, Crude extract, Antibacterial activity, Antifungal activity, Minimum inhibitory concentration
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How to cite this article:

Parimala, S., M. Kandhasamy and Chinnasamy, P.S. 2018. Antimicrobial Activity of the Crude Extract Derived from Streptomyces spp. Associated with Sponges.Int.J.Curr.Microbiol.App.Sci. 7(8): 1187-1194. doi: https://doi.org/10.20546/ijcmas.2018.708.133