National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Salmonella enterica serovar Typhi is an intracellular anaerobic gram-negative bacterium, which causes typhoid fever in humans. The emergence of multi-drug resistance Salmonella typhi strains has become a significant issue leading to more difficulties in the management of disease especially in developing countries. High genetic variations among the field isolates may cause vaccination failure. Therefore, there is a need of conserved and immunogenic proteins as vaccine candidate for S. typhi. Recent studies have shown that outer membrane proteins (Omps) of Salmonella have been considered as immunogens for eliciting active/protective immune response against Salmonella and thus, have great potential to act as possible vaccine candidates for typhoid. In present study, Outer membrane proteins of Salmonella typhi were isolated by ultracentrifugation method followed by quantification by Lowry method and recovery was found to be 30mg/lit approximately. Further Omps were analyzed by SDS-Polyacrylamide gel electrophoresis and nine proteins bands were observed ranging from small to medium size.
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