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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 2851-2855
DOI: https://doi.org/10.20546/ijcmas.2018.708.300


Isolation and Protein Profiling of Outer Membrane Proteins (Omps) of Salmonella typhi
Yashpal Singh1, Anjani Saxena2, Rajesh Kumar3 and M.K. Saxena4
1Department of Molecular Biology and Genetic Engg, College of Basic Science & Humanities, G.B. Pant University of Agriculture &Technology, Pantnagar, Uttarakhand, India
2Department of Pharmacology & Toxicology, 3Department of Veterinary Microbiology, 4Department of Veterinary Physiology and Biochemistry, College of Veterinary and Animal Sciences, G.B. Pant University of Agriculture &Technology, Pantnagar, Uttarakhand, India
*Corresponding author
Abstract:

Salmonella enterica serovar Typhi is an intracellular anaerobic gram-negative bacterium, which causes typhoid fever in humans. The emergence of multi-drug resistance Salmonella typhi strains has become a significant issue leading to more difficulties in the management of disease especially in developing countries. High genetic variations among the field isolates may cause vaccination failure. Therefore, there is a need of conserved and immunogenic proteins as vaccine candidate for S. typhi. Recent studies have shown that outer membrane proteins (Omps) of Salmonella have been considered as immunogens for eliciting active/protective immune response against Salmonella and thus, have great potential to act as possible vaccine candidates for typhoid. In present study, Outer membrane proteins of Salmonella typhi were isolated by ultracentrifugation method followed by quantification by Lowry method and recovery was found to be 30mg/lit approximately. Further Omps were analyzed by SDS-Polyacrylamide gel electrophoresis and nine proteins bands were observed ranging from small to medium size.


Keywords: Salmonella, Outer membrane proteins, vaccine
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How to cite this article:

Yashpal Singh, Anjani Saxena, Rajesh Kumar and Saxena, M.K. 2018. Isolation and Protein Profiling of Outer Membrane Proteins (Omps) of Salmonella typhi.Int.J.Curr.Microbiol.App.Sci. 7(8): 2851-2855. doi: https://doi.org/10.20546/ijcmas.2018.708.300