National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A heterotrophic strain ACM-1, isolated from Pangasius spp fish farms showed an ability to convert ammonium to nitrate under aerobic conditions. Based on the molecular phylogenetic analysis 16S rRNA gene sequence ACM-1 matched similarity to Achromobacter xylosoxidans (100%). ACM-1 showed amplification for Beta-proteobacterial 16S AOB gene (465 bp) confirming the characteristic feature of ammonia oxidation. Sodium pyruvate (1%) was confirmed as the probable carbon source for the luxurious growth of Achromobacter xylosoxidans among the six carbon sources tested. At 30oC, 5% preculture and 120 rpm in shaker, ACM-1 could able to remove 100% ammonium within 36h. The ammonium removal rate was reported to be 1.3 NH4+ -N mg L-1h-1 at 30oC. The rate of ammonium removal at different temperature (20oC, 25oC, 37oC) ranged between 0.9 to1.33 NH4+-N mg L-1 h-1. The nitrite produced during this experiment remained at constant rate and nitrate concentration increased after the initial hours of experiment. Therefore, the results demonstrate that Achromobacter xylosoxidans ACM-1 can remove ammonium in batch cultures and could be applied in aquaculture farms as a biological nitrifier.
Citations |
 |
 |
 |
 |
