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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 2645-2654
DOI: https://doi.org/10.20546/ijcmas.2018.708.273


Nitrification Potential of Achromobacter xylosoxidans Isolated from Fresh Water Finfish Farms of Kerala, India
Ahamed Basha*, Toms C. Joseph1, K.V. Lalitha1, D. Vineetha1, G. Rathore2, Gayatri Tripati3 and K. Pani Prasad3
1ICAR-Central Institute of Fisheries Technology, Cochin, Kerala -682 029, India
2ICAR-National Bureau of Fish Genetic Resources-Lucknow-226 002, India
3ICAR-Central Institute of Fisheries Education, Versova, Mumbai – 400061, India
*Corresponding author
Abstract:

A heterotrophic strain ACM-1, isolated from Pangasius spp fish farms showed an ability to convert ammonium to nitrate under aerobic conditions. Based on the molecular phylogenetic analysis 16S rRNA gene sequence ACM-1 matched similarity to Achromobacter xylosoxidans (100%). ACM-1 showed amplification for Beta-proteobacterial 16S AOB gene (465 bp) confirming the characteristic feature of ammonia oxidation. Sodium pyruvate (1%) was confirmed as the probable carbon source for the luxurious growth of Achromobacter xylosoxidans among the six carbon sources tested. At 30oC, 5% preculture and 120 rpm in shaker, ACM-1 could able to remove 100% ammonium within 36h. The ammonium removal rate was reported to be 1.3 NH4+ -N mg L-1h-1 at 30oC. The rate of ammonium removal at different temperature (20oC, 25oC, 37oC) ranged between 0.9 to1.33 NH4+-N mg L-1 h-1. The nitrite produced during this experiment remained at constant rate and nitrate concentration increased after the initial hours of experiment. Therefore, the results demonstrate that Achromobacter xylosoxidans ACM-1 can remove ammonium in batch cultures and could be applied in aquaculture farms as a biological nitrifier.


Keywords: Sediment, PCR, 16SrDNA, Achromobacter xylosoxidans, sodium pyruvate,               Hetertrophic nitrification
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How to cite this article:

Ahamed Basha, K., Toms C. Joseph, K.V. Lalitha, D. Vineetha1, G. Rathore, Gayatri Tripati and Pani Prasad, K. 2018. Nitrification Potential of Achromobacter xylosoxidans Isolated from Fresh Water Finfish Farms of Kerala, India.Int.J.Curr.Microbiol.App.Sci. 7(8): 2645-2654. doi: https://doi.org/10.20546/ijcmas.2018.708.273