National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Transgenic VRI-2 peanut plants were generated by following an Agrobacterium mediated, in planta transformation protocol with cry1AcF gene for efficacy against Spodoptera litura and Amsacta albistriga. Putative transgenics were selected by analyzing composite samples by GRID PCR method. The transgenic nature of the putative transgenics was further ascertained by molecular analysis for the presence of the gene of interest and selectable marker followed by efficacy against S. litura and/or Amsacta albistriga. The plants that showed 57.9-100 % mortality against S. litura were selected for further analysis. The stability of the selected T1 plants in the subsequent generation was confirmed by not only integration and efficacy but also expression by ELISA. Further, Southern analysis of T2 generation plants revealed the integration pattern of the transgene in the peanut genome and improved larval mortality in the range of 25-78.94%. The study demonstrates that overexpression of cry1AcF in peanut provide resistance to S. litura and A. albistriga.
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