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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 2567-2583
DOI: https://doi.org/10.20546/ijcmas.2018.708.264


A Synthetic cry1AcF Gene Imparts Resistance to Spodoptera litura (F.) and Amsacta albistriga Walker in Peanut (Arachis hypogaea L.) cv. VRI-2
Keshavareddy1*, A.R.V. Kumar1, Vemanna S. Ramu2, Rohini Sreevathsa3 and Udayakumar2
1Department of Agricultural Entomology, 2Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bengaluru-560065, Karnataka, India
3National Research Centre on Plant Biotechnology, New Delhi-110012, India
*Corresponding author
Abstract:

Transgenic VRI-2 peanut plants were generated by following an Agrobacterium mediated, in planta transformation protocol with cry1AcF gene for efficacy against Spodoptera litura and Amsacta albistriga. Putative transgenics were selected by analyzing composite samples by GRID PCR method. The transgenic nature of the putative transgenics was further ascertained by molecular analysis for the presence of the gene of interest and selectable marker followed by efficacy against S. litura and/or Amsacta albistriga. The plants that showed 57.9-100 % mortality against S. litura were selected for further analysis. The stability of the selected T1 plants in the subsequent generation was confirmed by not only integration and efficacy but also expression by ELISA. Further, Southern analysis of T2 generation plants revealed the integration pattern of the transgene in the peanut genome and improved larval mortality in the range of 25-78.94%. The study demonstrates that overexpression of cry1AcF in peanut provide resistance to S. litura and A. albistriga.


Keywords: Peanut, In planta, Transgenics, Spodoptera litura and Amsacta albistriga
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How to cite this article:

Keshavareddy, G., A.R.V. Kumar, Vemanna S. Ramu, Rohini Sreevathsa and Udayakumar, M. 2018. A Synthetic cry1AcF Gene Imparts Resistance to Spodoptera litura (F.) and Amsacta albistriga Walker in Peanut (Arachis hypogaea L.) cv. VRI-2.Int.J.Curr.Microbiol.App.Sci. 7(8): 2567-2583. doi: https://doi.org/10.20546/ijcmas.2018.708.264