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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 2562-2566
DOI: https://doi.org/10.20546/ijcmas.2018.708.263


Validation of a Simple and Rapid Method for Isolating Genomic DNA from Medicinal and Aromatic Plants for Subsequent Polymerase Chain Reaction
Vikash Kumar, Abhishek Prasad, Chandan Roy and Tirthartha Chattopadhyay*
Department of Plant Breeding and Genetics, Bihar Agricultural College, Bihar Agricultural University, Sabour, Bhagalpur, Bihar-813210, India
*Corresponding author
Abstract:

The robust technique of polymerase chain reaction (PCR) has revolutionized the field of plant molecular biology and has made the molecular characterization of crop plants easy, rapid and reproducible. Only a minute amount of input genomic DNA is required in PCR. But, the isolated DNA must be free from different contaminants, which can potentially act as PCR inhibitors. The efficiency of PCR might be reduced in case of the medicinal and aromatic plants, due to the presence of biomolecules acting as PCR inhibitors. Hence the present study reports the applicability of a simple and rapid method for isolating genomic DNA from medicinal and aromatic plants, which can be successfully applied for PCR-based genotyping of these plants. Using a modified form of the detergent (SDS)-potassium acetate method, genomic DNA from the mature leaf tissues of 7 different medicinal and aromatic plants, viz., slender dwarf morning-glory (Evolvulus alsinoides L.), horse mint (Mentha longifolia L.), centella (Centella asiatica L.), brahmi (Bacopa monnieri L.), ashwagandha (Withania somnifera L.), vasaka (Adhatoda vasica Nees.) and sarpgandha (Rauwolfia serpentina L.) was isolated and PCR was carried out with different inter short sequence repeat (ISSR) primers. Gel electrophoresis revealed the presence of distinguishable sharp amplicons, prompting us to advocate the utility of this method for genotyping of medicinal and aromatic plants.


Keywords: ISSR, Medicinal and aromatic plants, Polymerase chain reaction, Rapid DNA isolation, Secondary metabolites
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How to cite this article:

Vikash Kumar, Abhishek Prasad, Chandan Roy and Tirthartha Chattopadhyay. 2018. Validation of a Simple and Rapid Method for Isolating Genomic DNA from Medicinal and Aromatic Plants for Subsequent Polymerase Chain Reaction.Int.J.Curr.Microbiol.App.Sci. 7(8): 2562-2566. doi: https://doi.org/10.20546/ijcmas.2018.708.263