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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 775-782
DOI: https://doi.org/10.20546/ijcmas.2018.708.086


PCR-RFLP Analysis of TNP2 Gene in Indigenous Cattle Breeds
Ashish Ranjan1*, K.N. Raja1, Ranjana Sinha2 and I. Ganguly1
1Division of Animal Genetics and Breeding, NDRI, Karnal, 132001 (India)
2Livestock Production Management, NDRI, Karnal, 132001 (India) ICAR-National Dairy Research Institute, Karnal, Haryana-132001, India
*Corresponding author
Abstract:

Present study was conducted on 50 bulls and 40 male calves of Sahiwal, Tharparkar and Karan Fries cattle maintained at Artificial Breeding Research Center and Livestock Research Center, NDRI Karnal (Haryana) to identify genetic polymorphisms in TNP2 gene. A total of 1528 bp of TNP-2 gene were studied in Bos indicus cattle breeds, which are widely distributed in Indian sub-continent. Three sets of primers for TNP2 gene on the basis of Bos Taurus sequence (Acc. No- BK_006514) were designed using Primer3 software and PCR products of 459, 483, 484 base pairs were obtained. Amplicons were custom sequenced and subjected to Clustal W analysis which showed nucleotide changes in non coding region in Indian cattle breeds as compared to Bos taurus. Analysis of SNPs were performed, using restriction fragment length polymorphism (PCR-RFLP), to detect nucleotide changes in the sequence as reported (g. 480 C>T and g. 1536 C>T) in Chinese Holstein breed. Polymorphism at 480 C>T was present while for g. 1536 C>T, there were absence of variability in the sampled population of Sahiwal, Tharparkar and Karan Fries cattle.


Keywords: Indigenous cattle, Cladogram, Polymorphism, PCR-RFLP, TNP2 gene.
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How to cite this article:

Ashish Ranjan, Raja, K.N., Ranjana Sinha and Ganguly, I. 2018. PCR-RFLP Analysis of TNP2 Gene in Indigenous Cattle Breeds.Int.J.Curr.Microbiol.App.Sci. 7(8): 775-782. doi: https://doi.org/10.20546/ijcmas.2018.708.086