National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Effect of growth regulators on frequency of callus induction and shoot regeneration from anthers of two japonica rice varieties viz., Azucena and Moroberekan were studied. Anthers from panicles in which the distance between flag leaf and subtending leaf was 12-13 cm in Azucena and 14-15 cm in Moroberekan were selected for culture. At this stage of development, anthers contained mid-uninucleate pollen grains. Panicles were subjected to cold pre-treatment of 4°C for 8 days. Higher callus induction frequency 49.99 % and 48.64 % obtained on N6 medium containing 2 mg/L 2,4-D + 1 mg/L Kinetin (T4) and 1 mg/L 2,4-D + 2 mg/L NAA + 0.5 mg/L Kinetin (T11) however, higher shoot regeneration frequency 83.99 % and 80.00 % obtained on MS medium containing 0.5 mg/L Kinetin + 2 mg/L BAP (T10) + 1 mg/L NAA and 2 mg/L Kinetin + 1 mg/L BAP + 1 mg/L NAA (T16) in Azucena and Moroberekan respectively. The present results indicated the anther culture is influence by the genotypes and media composition and this investigation also can be used in the DH (Double Haploid) production in rice.
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