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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 667-677
DOI: https://doi.org/10.20546/ijcmas.2018.708.072


Indirect Regeneration of Japonica Rice (Oryza sativa L.) Varieties through Anther Culture
Avinash Sharma1*, Dalpat Lal1, Monoj Sutradhar1, Hansraj Pradhan2 and Nirupa Kumari3
1Department of Plant Biotechnology, University of Agricultural Sciences, GKVK, Bengaluru, Karnataka, India
2Department of Crop Improvement, CSKHPKV, Palampur, India
3Department of Botany, Patna University, India
*Corresponding author
Abstract:

Effect of growth regulators on frequency of callus induction and shoot regeneration from anthers of two japonica rice varieties viz., Azucena and Moroberekan were studied. Anthers from panicles in which the distance between flag leaf and subtending leaf was 12-13 cm in Azucena and 14-15 cm in Moroberekan were selected for culture. At this stage of development, anthers contained mid-uninucleate pollen grains. Panicles were subjected to cold pre-treatment of 4°C for 8 days. Higher callus induction frequency 49.99 % and 48.64 % obtained on N6 medium containing 2 mg/L 2,4-D + 1 mg/L Kinetin (T4) and 1 mg/L 2,4-D + 2 mg/L NAA + 0.5 mg/L Kinetin (T11) however, higher shoot regeneration frequency 83.99 % and 80.00 % obtained on MS medium containing 0.5 mg/L Kinetin + 2 mg/L BAP (T10) + 1 mg/L NAA and 2 mg/L Kinetin + 1 mg/L BAP + 1 mg/L NAA (T16) in Azucena and Moroberekan respectively. The present results indicated the anther culture is influence by the genotypes and media composition and this investigation also can be used in the DH (Double Haploid) production in rice.


Keywords: Anther, Japonica rice, Cold pre-treatment, Callus induction, Shoot regeneration
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How to cite this article:

Avinash Sharma, Dalpat Lal, Monoj Sutradhar, Hansraj Pradhan and Nirupa Kumari. 2018. Indirect Regeneration of Japonica Rice (Oryza sativa L.) Varieties through Anther Culture.Int.J.Curr.Microbiol.App.Sci. 7(8): 667-677. doi: https://doi.org/10.20546/ijcmas.2018.708.072