National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Proteases are one of the most important groups of enzymes with wide range of industrial applications. The soil isolate Bacillus cereusAG1 produced alkaline protease under submerged cultivation condition. Maximum production of enzyme was achieved at pH 10.0 and temperature 30oC at 120 rpm. The supplementation of fructose as carbon source and combination of beef extract, yeast extract and peptone as nitrogen source improved enzyme production by 1.39 times. Partial purification of enzyme by dialysis exhibited 3.07 fold purification with 60.15% yield. The characterization of partially purified alkaline protease displayed optimum activity at pH 10.0, temperature 50oC with casein as the most suitable substrate. Mmaximum enzyme activity 250 U ml-1and Michaelis constant (0.28%) of alkaline protease were obtained by varying casein concentration (1.0-2.0% w/v). The alkaline protease activity was enhanced in the presence of Ca+2, while Na+ and Hg+2 inhibited the enzyme activity. The enzyme retained 59.92% activity in the presence of 5.0 mM EDTA. The detergent stability and compatibility of alkaline protease suggest its potential utility as cleaning additive.
Citations |
 |
 |
 |
 |
