National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The objective of the study was to see the prevalence of C. perfringens as well as to detect the enterotoxigenic genes in C. perfringens strains isolated from different meat and water samples in Kashmir, India. For this 275 different meat and meat product, and water samples were analyzed by standard bacteriological technique. All the Fifty nine (59) isolates recovered from these samples were typed according to their gene by multiplex PCR assay. Out of these, 44 (74.57%) isolates were confirmed as C. perfringens type A based on the presence of alpha toxin gene (cpa). The highest number of type A isolates were recovered from raw mutton (08) followed by mutton kabab (07) and mutton curry (06). Eight isolates (18.60%) were classified as un-typable strains as no other gene was detected by PCR. To ascertain the prevalence of C. perfringens enterotoxin gene (cpe), all type A isolates were also screened for presence of cpe gene separately. From the PCR assay, it was found that 13 (29.55%) of C. perfringens type A isolates carried the cpe gene of 233 bp. Isolates recovered from chicken pickle showed highest occurrence (100%) of cpe gene followed by raw chicken (50%) and mutton kabab (42.86%). Strain having the cpe gene is of public health concern that is mainly responsible for causing food poisoning.
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