Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 480-489
DOI: https://doi.org/10.20546/ijcmas.2018.708.053


Enterotoxigenecity and Typing of Clostridium perfringens Isolated from Raw Meat, Meat Products and Water Samples in Kashmir, India
Syed Akram Hussain*, M.M. Willayat and Mudasir A. Rather
Division of Veterinary Public Health, Faculty of Veterinary Sciences and Animal Husbandry, SKUAST-K, Shuhama, Alusteng, Srinagar-190006, J&K, India
*Corresponding author
Abstract:

The objective of the study was to see the prevalence of C. perfringens as well as to detect the enterotoxigenic genes in C. perfringens strains isolated from different meat and water samples in Kashmir, India. For this 275 different meat and meat product, and water samples were analyzed by standard bacteriological technique. All the Fifty nine (59) isolates recovered from these samples were typed according to their gene by multiplex PCR assay. Out of these, 44 (74.57%) isolates were confirmed as C. perfringens type A based on the presence of alpha toxin gene (cpa). The highest number of type A isolates were recovered from raw mutton (08) followed by mutton kabab (07) and mutton curry (06). Eight isolates (18.60%) were classified as un-typable strains as no other gene was detected by PCR. To ascertain the prevalence of C. perfringens enterotoxin gene (cpe), all type A isolates were also screened for presence of cpe gene separately. From the PCR assay, it was found that 13 (29.55%) of C. perfringens type A isolates carried the cpe gene of 233 bp. Isolates recovered from chicken pickle showed highest occurrence (100%) of cpe gene followed by raw chicken (50%) and mutton kabab (42.86%). Strain having the cpe gene is of public health concern that is mainly responsible for causing food poisoning.


Keywords: C. perfringens type A, Enterotoxignecity, PCR, cpe gene, Meat & meat products
Download this article as Download

How to cite this article:

Syed Akram Hussain, M.M. Willayat and Mudasir A. Rather. 2018. Enterotoxigenecity and Typing of Clostridium perfringens Isolated from Raw Meat, Meat Products and Water Samples in Kashmir, India.Int.J.Curr.Microbiol.App.Sci. 7(8): 480-489. doi: https://doi.org/10.20546/ijcmas.2018.708.053