Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 452-471
DOI: https://doi.org/10.20546/ijcmas.2018.708.051


Molecular Cloning of Retinoic-Acid Metabolizing Cytochrome P450 26B1 (Cyp26B1) and Expression Analysis during Different Reproductive Phase in Clarias magur (Hamilton 1822)
Deepak Agarwal1, P. Gireesh-Babu1*, Vandita Kohli2, Om Pravesh K. Ravi3, Srijit Chakravarty3, Adnan Gora4 and Aparna Chaudhari1
1Fish Genetics and Biotechnology Division
2Fish Post Harvest Management Division, ICAR-Central Institute of Fisheries Education, Versova, Mumbai-400 061, India
3CoFS, BAU, Ranchi, India
4CMFRI, Kochi, India
*Corresponding author
Abstract:

Retinoid signalling is crucial to vertebrate embryonic development. The Cyp26B1 is one of the key catabolic enzymes, involved in the inactivation of all-trans retinoic acid and helps in the maintaining of retinoic acid availability for retinoid signalling. In the present study, for the first time, we cloned and characterized the full coding sequence of Cyp26B1 gene in Clarias magur. The full-length cDNA sequence of Cyp26B1 gene was 1782 bp (ORF 1533 bp) encoding 511 amino acids. Signal peptide analysis revealed that Cyp26B1 is a secretory protein as reported in other vertebrates. The hydropathy profile predicted that Cyp26B1 is hydrophilic in nature. The overall quality factors of Cyp26B1 protein by ERRAT analysis was 56.19%, indicating the instability of proteins. The expression profile generated for the preparatory, spawning, 6h and 16h post OvatideTM injection stages, revealed the significantly higher expression (P<0.05) of Cyp26B1 mRNA in the brain of female fish compared to the ovary at all developmental stages and peaked at spawning stage. Whereas in male fish, the mRNA expression was significantly higher at 16h post OvatideTM injection stages in the brain and minor expression obtained at all the stages of testis. Our findings suggest that maintenance and clearance of retinoic acid are essential at every developmental stage in C. magur and Cyp26B1 might play important role in germ cell development ultimately affect the reproductive physiology.


Keywords: Cyp26B1, Retinoic acid, Preparatory phase, OvatideTM, Retinoid signalling
Download this article as Download

How to cite this article:

Deepak Agarwal, P. Gireesh-Babu, Vandita Kohli, Om Pravesh K. Ravi, Srijit Chakravarty, Adnan Gora and Aparna Chaudhari. 2018. Molecular Cloning of Retinoic-Acid Metabolizing Cytochrome P450 26B1 (Cyp26B1) and Expression Analysis during Different Reproductive Phase in Clarias magur (Hamilton 1822).Int.J.Curr.Microbiol.App.Sci. 7(8): 452-471. doi: https://doi.org/10.20546/ijcmas.2018.708.051