National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Retinoid signalling is crucial to vertebrate embryonic development. The Cyp26B1 is one of the key catabolic enzymes, involved in the inactivation of all-trans retinoic acid and helps in the maintaining of retinoic acid availability for retinoid signalling. In the present study, for the first time, we cloned and characterized the full coding sequence of Cyp26B1 gene in Clarias magur. The full-length cDNA sequence of Cyp26B1 gene was 1782 bp (ORF 1533 bp) encoding 511 amino acids. Signal peptide analysis revealed that Cyp26B1 is a secretory protein as reported in other vertebrates. The hydropathy profile predicted that Cyp26B1 is hydrophilic in nature. The overall quality factors of Cyp26B1 protein by ERRAT analysis was 56.19%, indicating the instability of proteins. The expression profile generated for the preparatory, spawning, 6h and 16h post OvatideTM injection stages, revealed the significantly higher expression (P<0.05) of Cyp26B1 mRNA in the brain of female fish compared to the ovary at all developmental stages and peaked at spawning stage. Whereas in male fish, the mRNA expression was significantly higher at 16h post OvatideTM injection stages in the brain and minor expression obtained at all the stages of testis. Our findings suggest that maintenance and clearance of retinoic acid are essential at every developmental stage in C. magur and Cyp26B1 might play important role in germ cell development ultimately affect the reproductive physiology.
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