National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Escherichia coli are important pathogens that cause serious infections in hospitalized patients. This study aimed to investigate the occurrence of β-lactamase genes in twelve ESBL-producing E. coli isolated from patients in the Santa Casa de Misericordia de Sobral-CE, a teaching hospital in Northeastern Brazil, and analyze the antimicrobial susceptibility profile of these microorganisms. Genetic detection of the blaCTX-M, blaSHV, and blaTEM genes was performed by PCR. All isolates were susceptible to ertapenem and meropenem, 11 (92%) were sensitive to amikacin, 9 (75%) to colistin, imipenem and tigecycline. Moreover, 92% were resistant to ceftriaxone, cefuroxime, cefuroxime/axetil, and 100% to ampicillin and ciprofloxacin. The blaCTX-M gene was detected in 9 (75%) isolates, blaSHV in 6 (50%), and blaTEM in 5 (41.6%). In 3 (25%) of the isolates were detected simultaneously genes encoding all three types of β-lactamase investigated. Additionally, the antimicrobial activity of α-pinenne, β- citronellol, Ipomoea carnea and Ipomoea asarifolia extracts against E. coli ATCC 25992 was analyzed, but there was no antimicrobial activity. However, more studies are needed to verify the spread of these organisms in the hospital environment and to evaluate the antimicrobial activity of new compounds and herbal extracts against multidrug resistant pathogens involved in nosocomial infections.
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