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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 422-434
DOI: https://doi.org/10.20546/ijcmas.2018.708.048


Antimicrobial Susceptibility and Genetic Characterization of Extended Spectrum Beta-Lactamase (ESBL)-Producing Nosocomial Strains of Escherichia coli Isolated from a Brazilian Teaching Hospital
Rafaele Aragão dos Santos, Vicente de Paulo Teixeira Pinto and Francisco Cesar Barroso Barbosa*
Microbiology Laboratory, FAMED, Federal University of Ceará, Avenida Comandante Maurocélio Rocha Pontes 100, 62.042-280 Sobral, Ceará, Brazil
*Corresponding author
Abstract:

Escherichia coli are important pathogens that cause serious infections in hospitalized patients. This study aimed to investigate the occurrence of β-lactamase genes in twelve ESBL-producing E. coli isolated from patients in the Santa Casa de Misericordia de Sobral-CE, a teaching hospital in Northeastern Brazil, and analyze the antimicrobial susceptibility profile of these microorganisms. Genetic detection of the blaCTX-M, blaSHV, and blaTEM genes was performed by PCR. All isolates were susceptible to ertapenem and meropenem, 11 (92%) were sensitive to amikacin, 9 (75%) to colistin, imipenem and tigecycline. Moreover, 92% were resistant to ceftriaxone, cefuroxime, cefuroxime/axetil, and 100% to ampicillin and ciprofloxacin. The blaCTX-M gene was detected in 9 (75%) isolates, blaSHV in 6 (50%), and blaTEM in 5 (41.6%). In 3 (25%) of the isolates were detected simultaneously genes encoding all three types of β-lactamase investigated. Additionally, the antimicrobial activity of α-pinenne, β- citronellol, Ipomoea carnea and Ipomoea asarifolia extracts against E. coli ATCC 25992 was analyzed, but there was no antimicrobial activity. However, more studies are needed to verify the spread of these organisms in the hospital environment and to evaluate the antimicrobial activity of new compounds and herbal extracts against multidrug resistant pathogens involved in nosocomial infections.


Keywords: Antimicrobial activity, bla genes, Escherichia coli, ESBL, α-pinene, β-citronellol, Ipomoea carnea, Ipomoea asarifolia, Teaching hospital
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How to cite this article:

Rafaele Aragão dos Santos, Vicente de Paulo Teixeira Pinto and Francisco Cesar Barroso Barbosa. 2018. Antimicrobial Susceptibility and Genetic Characterization of Extended Spectrum Beta-Lactamase (ESBL)-Producing Nosocomial Strains of Escherichia coli Isolated from a Brazilian Teaching Hospital.Int.J.Curr.Microbiol.App.Sci. 7(8): 422-434. doi: https://doi.org/10.20546/ijcmas.2018.708.048