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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 329-338
DOI: https://doi.org/10.20546/ijcmas.2018.708.037


Isolation, Morphological and Cultural Characterization of Azospirillum Isolated from Rhizospheric Soils of Various Non-Leguminous Crops of Ranchi Having Acidic pH
R. Narayan*, N.C. Gupta and D.K. Shahi
Department of Soil Science and Agricultural Chemistry, Birsa Agricultural University, Ranchi, Jharkhand, India
*Corresponding author
Abstract:

Azospirillum is one of the versatile non-symbiotic, free living diazotrophic bacteria which appears to have a world-wide distribution and occurs in large number in the rhizosphere soil of a variety of grasses and cereals. The present study was carried out during Rabi and Kharif 2016-17 in the Department of Soil Science and Agricultural Chemistry, Birsa Agricultural University, Ranchi, Jharkhand. Efforts were made to screen out the presence of Azospirillum in rhizosphere of various non-leguminous crops and to characterize the isolates on the basis of morphological and cultural behaviours. On the basis of pH range (4.0-5.5), 54 rhizospheric soil samples were tentatively selected out of 100 samples for investigation. From the study conducted, presence of Azospirillum in rhizosphere of acidic pH was confirmed. Morphological characterization revealed that Azospirillum isolated from rhizosphere of various crops were gram negative and vibroid in shape. Cells were encapsulated i.e., were having capsules around them and formed microcyst in aged culture. Cultural characterisation revealed that colonies developed on agar slants were smooth, some of them were having raised while others were having flat elevation. Amount of growth of colonies observed were dense in 43 and thin in 11 colonies while they developed white sub-surface pellicle when grown in semi-solid Okon’s media. Out of 54 colonies, 41 were white, 5 were red and rest colonies were found yellow in colour.


Keywords: Azospirillum, Rhizosphere, Diazotrophic bacteria, Isolation, Characterisation, Microcyst, Capsule
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How to cite this article:

Narayan, R., N.C. Gupta and Shahi, D.K. 2018. Isolation, Morphological and Cultural Characterization of Azospirillum Isolated from Rhizospheric Soils of Various Non-Leguminous Crops of Ranchi Having Acidic pH.Int.J.Curr.Microbiol.App.Sci. 7(8): 329-338. doi: https://doi.org/10.20546/ijcmas.2018.708.037