National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Azospirillum is one of the versatile non-symbiotic, free living diazotrophic bacteria which appears to have a world-wide distribution and occurs in large number in the rhizosphere soil of a variety of grasses and cereals. The present study was carried out during Rabi and Kharif 2016-17 in the Department of Soil Science and Agricultural Chemistry, Birsa Agricultural University, Ranchi, Jharkhand. Efforts were made to screen out the presence of Azospirillum in rhizosphere of various non-leguminous crops and to characterize the isolates on the basis of morphological and cultural behaviours. On the basis of pH range (4.0-5.5), 54 rhizospheric soil samples were tentatively selected out of 100 samples for investigation. From the study conducted, presence of Azospirillum in rhizosphere of acidic pH was confirmed. Morphological characterization revealed that Azospirillum isolated from rhizosphere of various crops were gram negative and vibroid in shape. Cells were encapsulated i.e., were having capsules around them and formed microcyst in aged culture. Cultural characterisation revealed that colonies developed on agar slants were smooth, some of them were having raised while others were having flat elevation. Amount of growth of colonies observed were dense in 43 and thin in 11 colonies while they developed white sub-surface pellicle when grown in semi-solid Okon’s media. Out of 54 colonies, 41 were white, 5 were red and rest colonies were found yellow in colour.
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