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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(9): 530-539
DOI: http://dx.doi.org/10.20546/ijcmas.2016.509.059


Investigation of the Action of the Anolyte after Different Storage Times on the Gram-negative Bacteria
Teodora P. Popova1*, Toshka Petrova1 and Stoil Karadzhov2
1University of Forestry, Faculty of Veterinary Medicine, 10 Kl, Ohridski Blvd., Sofia 1756, Bulgaria
2Bulgarian Association of Activated Water, Kutuzov blvd, 39, 1619 Sofia, Bulgaria  
*Corresponding author
Abstract:

Studies were carried out to determine the sensitivity of pathogenic Gram-negative bacteria (Salmonella enterica ATCC and Pseudomonas aeruginosa strain №450) to electrochemically activated 3% aqueous sodium chloride solution (anolyte) in concentrations of 100%, 50%, 25% and 12,5% immediately after preparation (fresh) and after storage at room temperature for 7 days, 21 days and 28 days. As a control was used the disinfectant Virkon S in concentrations of 1%, 0,5%, 0,25% and 0,125%. It was found that the suspension of P. aeruginosa with density of 106 cells/ml was killed after 2 minutes under the influence of all tested concentrations of the fresh anolyte. The same strain in concentration of 108 cells/ml was killed within 2 minutes by 50% and 100% fresh anolyte, but the lower concentrations had such effect after 5 - 10 minutes. The anolyte storaged 7 days had similar action. After 21 and 28 days the anolyte in all tested concentrations had fast bactericidal activity on P. aeruginosa in suspension of 106 cells/ml (in 2 minutes), but in such of 108 cells/ml single cells remained viable even after 10 minutes. S. enterica in both tested suspensions - of 106 and 108 cells/ml was inactivated within 2 minutes by all examined concentrations not only of the fresh anolyte, but of these stored for 7, 21 and 28 days. Virkon S in all tested concentrations killed within two minutes P. aeruginosa and S. enterica in both examined densities.


Keywords: Electrochemically, sodium chloride, anolyte, Pseudomonas aeruginosa, Salmonella enterica, antibacterial activity.
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How to cite this article:

Teodora P. Popova, Toshka Petrova and Stoil Karadzhov. 2016. Investigation of the Action of the Anolyte after Different Storage Times on the Gram-negative Bacteria.Int.J.Curr.Microbiol.App.Sci. 5(9): 530-539. doi: http://dx.doi.org/10.20546/ijcmas.2016.509.059