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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 10-17
DOI: https://doi.org/10.20546/ijcmas.2018.708.002


Screening of Bacteria for Their Chlorpyrifos Degrading Ability
Uunati N. Ahir, Trupti K. Vyas*, Bhavik P. Sutaria, Priti R. Faldu and K.G. Patel
Food Quality Testing Laboratory, N M College of Agriculture, Navsari Agricultural University, Navsari – 396450, Gujarat, India
*Corresponding author
Abstract:

Chlorpyrifos (0, 0 – diethyl 0 - (3, 5, 6 – trichloro – 2 - pyridyl phosphorothioate) is broadspectrum pesticide with trade names Dursban, Lorsban and Spannit, displaying insecticidal activity against Lepidoptera, Diptera, Homoptera, Coleoptera, Orthoptera, Hymenoptera and Hemiptera. However, these organophosphates pesticides have acute neurotoxicity as they suppress acetylcholine esterase (AChE). Hence, present study deals with exploring bacteria for chlorpyrifos degradation ability. Total 10 different samples were collected from soil where chlorpyrifos pesticide was applied. Out of these 10 samples, six samples showed bacteria which are able to grow on Bushnell and Haas agar containing chlorpyrifos as sole carbon source. 15 bacteria which produce zone of clearance near colony were considered efficient chlorpyrifos degrader and purified for further studies. Screening of the isolate for chlorpyrifos revealed that AUC10 showed higher degradation (95 %), followed by AUC12 (93 %) and AUC11 (90 %). Ability of isolates to emulsify hydrophobic molecules revealed that higher emulsification activity was found in groundnut oil followed by coconut oil and cotton seed oil. Morphological characterization revealed that out of 15 isolates 6 bacteria were Gram positive whereas 9 isolates were Gram negative. Thus, isolate AUC10 possessed higher chlorpyrifos degradation rate compared to other isolated bacteria and can be used for bioremediation of chlorpyrifos contaminated sites.


Keywords: Biodegradation, Chlorpyrifos, Emulsification activity, Pesticide, Organophosphate
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How to cite this article:

Uunati N. Ahir, Trupti K. Vyas, Bhavik P. Sutaria, Priti R. Faldu and Patel, K.G. 2018. Screening of Bacteria for Their Chlorpyrifos Degrading Ability.Int.J.Curr.Microbiol.App.Sci. 7(8): 10-17. doi: https://doi.org/10.20546/ijcmas.2018.708.002