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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(9): 489-495
DOI: http://dx.doi.org/10.20546/ijcmas.2016.509.054


Molecular Distinction of Algae using Molecular Marker
B.K. Chikkaswamy and Rabin Chandra Paramanik*
Om Bio Science Research Centre, Indiranagar, Bangalore, India
*Corresponding author
Abstract:

RAPD molecular method is the Expressed Sequence database was used as the original genomic information for identification of polymorphic sites. Primers OPW-04, 05 and 06 RAPD is designed and the amplified PCR products are then directly sequenced. All sequences obtained are aligned to search possible RAPD altering the restriction enzymes recognition sites. At last, the method is used to genotype of these RAPD markers in large population samples of the of chlorococcales species complex. The physico-chemical parameters like phosphate and nitrate exhibit a positive correlation with the dynamics of chlorococcales. The results showed that 70, 69 and 75 RAPD bands with Primer OPW-0showed geographical discrimination. Molecular markers in algae and can be more relevant than other neutral markers mentioned above due to the high occurrence of RAPD in their functional genomic regions. Because of the potential for higher genotyping efficiency, data quality, genome-wide coverage, and analytical simplicity, RAPD have been used as molecular markers in evolutionary and ecological studies of a wide range of organisms in the current study, we report the characterization of 19 species of ofchlorococcales4, OPW-05 and OPW- 06are useful for genetic diversity studies of chlorococcales as they.


Keywords: Genetic diversity,chlorococcales, RAPD,strains, geographical region.
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How to cite this article:

Chikkaswamy, B.K., and Rabin Chandra Paramanik. 2016. Molecular Distinction of Algae using Molecular Marker.Int.J.Curr.Microbiol.App.Sci. 5(9): 489-495. doi: http://dx.doi.org/10.20546/ijcmas.2016.509.054