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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(9): 617-625
DOI: http://dx.doi.org/10.20546/ijcmas.2016.509.070


Identification of Bacteria that Contaminate Stored Streptomycin Species at 4°C
Aurpita Shaha1,2*, Ariful Haque3, Uzzal Haque4 and Anwar Ul Islam2
1Department of Molecular Pharmacology, Institute of Biomedical sciences,Tokushima University Graduate School, Tokushima, Japan
2Department of Pharmacy, Rajshahi University, Rajshahi-6205, Bangladesh
3Institute of Biological Science, Rajshahi University, Rajshahi, Bangladesh
4Department of Pharmacy, Jessore University of Science and Technology
*Corresponding author
Abstract:

The present study aims at exploring the bacteria that contaminate potential antibiotics producing indigenous marine Streptomyces species that stored at 4°C 4. Three organisms isolated from stored Streptomyces strain designated as AIAS-5, AIAS-10 and AIAS-39was subjected for taxonomic characterization and identification as they have moderate to high antibacterial activity. It was found that the strain was closely resembled to Bacillus sp. This identification was further confirmed by the 16S rDNA sequence analysis. The result of BLAST search and phylogenetic analysis showed that the strain AIAS-5 was a type strain of Bacillus pumilus SAFR-032 with 98% similarity as it formed a distinct branch with other strains of this species; AIAS-10 shows 99% similarity with Bacillus amyloliquefaciens, phylogenetic tree build from the forward sequence of this strain from a distinct with separate branch in the tree and Strain AIAS-39 was identified as Bacillus toyonensis with 97% similarity. Strains AIAS-39 may be identified as a new species of Bacillus species as according to 1% difference in sequence similarity is needed to warrant a new species name. To confirm it is a new species further work on its phenotypic characteristics is going on.


Keywords: Bacterial culture, contamination, antibiotic, phylogenetic tree, Streptomycessp., Bacillus sp.
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How to cite this article:

Aurpita Shaha, Ariful Haque, Uzzal Haque and Anwar Ul Islam. 2016. Identification of Bacteria that Contaminate Stored Streptomycin Species at 4°C.Int.J.Curr.Microbiol.App.Sci. 5(9): 617-625. doi: http://dx.doi.org/10.20546/ijcmas.2016.509.070