National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Present investigation was carried out to select the resistant of amongst the mapping population lines through marker assisted selection. The investigation was carried out in the laboratory of Department of Plant Pathology and Biotechnology Centre, PGI, Dr. PDKV, Akola. The 30 chickpea genotypes were selected for screening from three mapping population of different crosses (JG 62(Susceptible) X ICCV 08113, PG 04305 (resistant) X JG 62 (susceptible) and PG 07101 (resistant) X JG 62 (susceptible). 10 genotypes from each population were selected. Marker assisted characterization of 30 chickpea genotypes differing for Fusarium wilt reaction was carried out using four molecular markers reported by earlier workers linked to disease resistant/susceptibility. In the present study, three different markers (namely, CS-27F/R 700bp, A07C 417bp and UBC-8251200bp) linked to susceptibility and three microsatellite based markers (TA-59 258bp) linked to resistance allele were validated. The molecular markers ASAP (CS27F/R 700bp), RAPD (A07C 417bp), ISSR (UBC-825 1200bp) and STMS (TA59 258bp) amplified against 30 genotypes of chickpea (F2 population). The results indicates that amongst 30 genotypes from the mapping populations 8 genotypes were resistant from cross I, 1 genotype from cross II and 7 genotypes from cross III shows amplification in CS27F/R 700bp. Other than theses genotypes bands are present in STMS primer, TA59 258bp which is linked to resistance to Fusarium wilt.
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