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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(9): 462-467
DOI: http://dx.doi.org/10.20546/ijcmas.2016.509.050


Bioprospecting of Endophytic Bacteria from Zingiber officinale with Antibacterial Activities
Rohini Sabu and E.K. Radhakrishnan*
School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala, India
*Corresponding author
Abstract:

Endophytes contribute a major role in biodiversity. They are well known for its uniqueness and antimicrobial properties. In our study endophytic bacteria were isolated from surface sterilized rhizome of Zingiber officinale. Sodium hypochlorite and ethanol were used for surface sterilization. Endophytic bacteria were isolated and purified. DNA of the isolates was amplified by 16 S rDNA and was identified. All the five of them belonged to Bacillus. These isolates were screened for antibacterial activity against a panel of five human pathogens namely Bacillus subtilis, Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus and Salmonella typhi by perpendicular streaking. All of the endophytic bacteria except one showed prominent antibacterial activity. Methanolic extracts of these isolates also confirmed potential antibacterial activity.


Keywords: Endophytic bacteria,Zingiber officinale, 16S rDNA, antibacterial activity.
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How to cite this article:

Rohini Sabu and E.K. Radhakrishnan. 2016. Bioprospecting of Endophytic Bacteria from Zingiber officinale with Antibacterial Activities.Int.J.Curr.Microbiol.App.Sci. 5(9): 462-467. doi: http://dx.doi.org/10.20546/ijcmas.2016.509.050