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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(9): 431-440
DOI: http://dx.doi.org/10.20546/ijcmas.2016.509.046


Molecular Characterization of 16S rRNA and Internal Transcribed Spacer (ITS) Regions of Aeromonas spp. Isolated from Cultured Freshwater Fishes in Malaysia 
K.P. Diyana-Nadhirah1 and M.Y. Ina-Salwany1,2*
1Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400,Serdang, Selangor, Malaysia
2Laboratory of Marine Biotechnology, Institute of Bioscience, Universiti Putra Malaysia,43400 Serdang, Selangor, Malaysia
*Corresponding author
Abstract:

The Aeromonas spp., which are found mostly in freshwater fishes are known to cause uncontrolled disease outbreak in aquaculture system. In the present study, twenty-two of Aeromonas spp. strains isolated from diseased freshwater fishes were identified using the 16S ribosomal RNA (rRNA) and internal transcribed spacer (ITS) regions gene amplification where they produced a 1500 bp and 1000-1200 bp amplicon each. Phylogenetic analysis with BLASTn and 16S rRNA neighbor-joining bootstrapping methods revealed that all strains were identified up to genus level with 15 strains were classified as Aeromonas hydrophila (A. hydrophila) and the rest were Aeromonas veronii (A. veronii). Further ITS gene analysis disclosed strains identification up to species level; A. hydrophila (14 strains) and A. veronii (8 strains). These rapid DNA-based detection methods are simple, easy to perform and faster in identifying Aeromonas spp., thus are absolutely efficient for regular monitoring of Aeromonas spp. in a potential outbreak situation.


Keywords: Aeromonas spp., PCR, 16S rRNA gene, ITS gene, neighbor-joining, Phylogenetic analysis.
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How to cite this article:

Diyana-Nadhirah, K.P., and M.Y. Ina-Salwany. 2016. Molecular Characterization of 16S rRNA and Internal Transcribed Spacer (ITS) Regions of Aeromonas spp. Isolated from Cultured Freshwater Fishes in Malaysia.Int.J.Curr.Microbiol.App.Sci. 5(9): 431-440. doi: http://dx.doi.org/10.20546/ijcmas.2016.509.046