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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(9): 302-310
DOI: http://dx.doi.org/10.20546/ijcmas.2016.509.034


Molecular Characterization Indian Isolates of Pasteurella multocida Isolated from Buffalo and Cattle in India
Archana Yadav1, Anita Sharma1, Rajesh Kumar2, V.D.P. Rao2 and M.K. Saxena3*
1Department of Microbiology, C.B.S.H,
2Department of Veterinary Microbiology, C.V.Sc
 3Animal Biotechnology Centre, Department of Veterinary Physiology and BiochemistryCollege of Veterinary and Animal Science, G. B. Pant University of Agriculture &Technology, Pantnagar-263145, India  
*Corresponding author
Abstract:

The applicability of   repetitive palindromic sequences based differentiation of Pasteurella multocida serogroup B isolates involved in outbreak of haemorrhagic septesemia (HS) in Tarai region of India was evaluated. Seven isolates of Pasteurella multocida B: 2 were isolated from buffaloes and cattle. These were subjected to phenotypic and genotypic characterization. PCR assays were used for the characterization of these isolates. Repetitive extragenic palindromic REP- PCR and Enterobacterial repetitive intergenic consensus ERIC- PCR differentiated all the seven isolates into six profiles. All the isolates were found genetically distinct from standard Pasteurella multocida strain P52 (Vaccine strain of India).Two isolates shared same profile while all other isolates shown different profiles. This study provides a clear evidence of presence of more than one isolate in single outbreak of HS and also provide indication of high  genetic variation among field isolates of Pasteurella and may be the reason of vaccine failure and outbreaks.


Keywords: Pasteurella multocida,palindromic REP- PCR Haemorrhagic Septicaemia
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How to cite this article:

Archana Yadav, Anita Sharma, Rajesh Kumar, V.D.P. Rao and M.K. Saxena. 2016.  Molecular Characterization Indian Isolates of Pasteurella multocida Isolated from Buffalo and Cattle in India.Int.J.Curr.Microbiol.App.Sci. 5(9): 302-310. doi: http://dx.doi.org/10.20546/ijcmas.2016.509.034