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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here
ICV 2022: 95.28
Index Copernicus ICI Journals Master List 2022 - IJCMAS--ICV 2022: 95.28
For more details click here
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Original Research Articles                      Volume : 5, Issue:9, September, 2016
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
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Int.J.Curr.Microbiol.App.Sci.2016.5(9): 282-289
DOI: http://dx.doi.org/10.20546/ijcmas.2016.509.032


Diagnosis of Larval Stage (Metacercaria) of Two Eye Trematodes from Some Local Fishes by using Polymorphic Random Amplified DNA (PCR-RAPD)
Abdul Hussein H. Awad* and Hajer R. Manhel
Biology Department College of Education for Pure Science Basrah University Basrah, Iraq  
*Corresponding author
Abstract:

Genetic variations were found between the larval stage of Diplostomum spathaceum and Diplostomum spp. using Random Amplified Polymorphic DNA (PCR-RAPD). Nine primers each of which is composed of ten base oligonucleotid were selected. The number of bands were different, It ranged from 2-9. Both types of trematodes were distinguished by using this technique.


Keywords: Diplostomum spathaceum,PCR-RAPD.

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How to cite this article:

Abdul Hussein H. Awad and Hajer R. Manhel. 2016. Diagnosis of Larval Stage (Metacercaria) of Two Eye Trematodes from Some Local Fishes by using Polymorphic Random Amplified DNA (PCR-RAPD).Int.J.Curr.Microbiol.App.Sci. 5(9): 282-289. doi: http://dx.doi.org/10.20546/ijcmas.2016.509.032
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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