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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(9): 174-181
DOI: http://dx.doi.org/10.20546/ijcmas.2016.509.019


Bacterial Colonization and Biofilm Formation among Diabetic Patients: A Therapeutic Challenge
Pallavi Sayal1*, Pushpa Devi2 and Kanwardeep Singh2
1Department of Microbiology, BPS, GMC (W), Khanpurkalan, Sonepat, Haryana, India
2Department of Microbiology, Govt Medical College, Amritsar, India
*Corresponding author
Abstract:

Recent advancements in technology has brought to use, plethora of implants or devices made of inert metals, plastics and other synthetic products. Unfortunately, adhesion and attraction of the bacteria to these surfaces made them extremely prone to bacterial biofilm formation. Effects of diabetes, promotes bacterial colonization and allows bacteriuria to easily extend resulting in urosepsis. Present study is designed to provide insight into bacterial colonization and biofilm formation among diabetic patients with indwelling urinary catheters. This prospective study was done on 189 randomly selected urinary catheterized diabetic patients with significant bacteriuria (symptomatic and asymptomatic). Semi-quantitative bacterial culture was performed and isolates were identified by standard biochemical tests. Biofilm formation was Tissue Culture Plate Method. One hundred and two (53.96%) patients had asymptomatic bacteriuria (ASB) and 87/189(46.03%) had urinary tract infection (UTI). Females (57.84%) were frequently seen with ASB than men (42.15%) and ASB was more common in the age group between 46 and 60 years (27.2%).Urinary tract infections on the other hand were more common in males (65.51%) than in females (34.48%).Escherichia coli was most common isolate 59/189(31.12%) followed by Klebsiella spp 50/189(26.45%). Biofilm formation was observed among 140/189 (74.07%) isolates, out of which 55/189(29.10%) were high, 85/189(44.97%) were moderate and 49/189(25.92%) were non biofilm producers. Indwelling bladder catheters are commonly deployed prosthetic medical devices, unfortunately they also provide access for bacteria from contaminated external environment into a vulnerable body cavity. Biofilm-like pods explains how bladder infections can persist in the face of robust host defense. Therefore, very dynamic research activity in the biofilm field is required.


Keywords: Bacterial Colonization and Biofilm Formation,Diabetic Patients
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How to cite this article:

Pallavi Sayal, Pushpa Devi and Kanwardeep Singh. 2016. Bacterial Colonization and Biofilm Formation among Diabetic Patients: A Therapeutic ChallengeInt.J.Curr.Microbiol.App.Sci. 5(9): 174-181. doi: http://dx.doi.org/10.20546/ijcmas.2016.509.019