Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(9): 44-53
DOI: http://dx.doi.org/10.20546/ijcmas.2016.509.006


Rapid Detection of Mutations in rpoB Gene of Rifampicin Resistant Mycobacterium tuberculosis Strains by Line Probe Assay in GGG Hospital, Jamnagar, Gujarat, India
Neela C. Katara*, Bindiya J. Ghedia and Mala Sinhaa
Department of Microbiology, Shree M. P. Shah Government Medical College and GGG Hospital, Jamnagar, Gujarat, India  
*Corresponding author
Abstract:

Multidrug resistant (MDR) tuberculosis, resistance to at least rifampicin (RMP) and isoniazid (INH), is an increasing problem both in the developed as well as in the developing countries. The early diagnosis of tuberculosis and the rapid detection of resistance to the major anti-tubercular drugs is therefore, of utmost importance for the effective control of the resurgent epidemic. This study was carried out to detect rpo B gene mutations in rifampicin resistant Mycobacterium tuberculosis isolated from MDR suspected sputum samples by line prob assay method. Total 3,346 MDR suspected (Criteria A,B,C) sputum samples were received in TB Culture -DST laboratory, Microbiology department, Guru Govind Singh Hospital, Jamnagar during study period for 6 months from January 2015 to may 2015 and proceeded for direct smear examination, DNA extraction, Amplification and Hybridization and detection of rpo B gene mutations and rifampicin resistance by line probe assay method. Out of 3,346 samples, 2210 sputum samples were showing direct smear positive for acid fast bacilli suggestive of Mycobacterium tuberculosis. Out of 2210 direct smear positive samples, total 141 sputum samples were rifampicin resistant M .tuberculosis and studied for presence of rpo B gene mutations by line probe assay method were most common wild type 8 were 77(54.6%) absent and mutant type 3 were 104(74.0%) present. The present study has shown detection of rpoB gene mutations in rifampicin resistance M.tuberculosis is helpful to epidemiological purpose to prevent primary treatment failure,to study success rate and detection rate of various geographical area.


Keywords: Diagnosis of rifampicin resistance,line probe assay
Download this article as Download

How to cite this article:

Neela C. Katara, Bindiya J. Ghedia and Mala Sinha. 2016. Rapid Detection of Mutations in rpoB Gene of Rifampicin Resistant Mycobacterium tuberculosis Strains by Line Probe Assay in GGG Hospital, Jamnagar, Gujarat, India.Int.J.Curr.Microbiol.App.Sci. 5(9): 44-53. doi: http://dx.doi.org/10.20546/ijcmas.2016.509.006