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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(5): 3219-3223
DOI: https://doi.org/10.20546/ijcmas.2018.705.376


Exploring the Role of Glucose in Optimizing In-Vitro Growth of Bacterial Isolates under Aluminium Stressed Conditions
Priyanka Arora1, Vipin Shukla2 and Geeta Singh3*
1School of Sciences, Noida International University, G.B Nagar, Greater Noida, U.P, India
2Department of Biotechnology, C.C.S University, Meerut, U.P., India
3Division of Microbiology, Indian Agricultural Research Institute (IARI), New Delhi, India

*Corresponding author
Abstract:

Aluminium is one of the most abundant metals. This metal is responsible for inhibiting growth and productivity of many cultures thereby affecting the metabolism of most of the microbes. The current study is aimed at examining the effect of glucose addition on bacterial growth under aluminium stressed conditions. For this purpose, nutrient broth was supplemented with different concentration of aluminium viz. 0mM, 20mM, 60mM and 80mM. In addition to above heavy metal supplementation, the nutrient broth was additionally provided with varying concentration of glucose (0.25%, 0.5% and 1% glucose). The Bacillus and Burkholderia cultures were allowed to grow in all possible combinations of provided aluminium and glucose concentrations. In case of Bacillus culture, when media was supplemented with 0.5% glucose, the culture growth was increased upto 2.0-folds in 20mM aluminium toxic conditions whereas growth of Burkholderia strain increased upto 5.0-folds and 8.0-folds in case of 20mM and 60mM aluminium stressed conditions respectively. The results conclude that under aluminium toxic conditions, 0.5% glucose supplementations could prove to be beneficial for bacterial optimal growth.


Keywords: Aluminium, Bacillus, Burkholderia, Glucose, Stress
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How to cite this article:

Priyanka Arora, Vipin Shukla and Geeta Singh. 2018. Exploring the Role of Glucose in Optimizing In-Vitro Growth of Bacterial Isolates under Aluminium Stressed Conditions.Int.J.Curr.Microbiol.App.Sci. 7(5): 3219-3223. doi: https://doi.org/10.20546/ijcmas.2018.705.376