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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(5): 3449-3457
DOI: https://doi.org/10.20546/ijcmas.2018.705.399


A Cross Sectional Study for Prevalence of Intestinal Parasitic Infestation by Using Saline, Iodine, Glycerol-Iodine, KOH and LPCB Wet Mount Preparations of Stool Samples From Patients Attending Aims, Dewas
Khyati Jain, Arjun S. Gurjar* and Madhurendra S. Rajput
Department of Microbiology, Amaltas Institute of Medical Sciences, Dewas, M.P., India
*Corresponding author
Abstract:

The complexity of health ailments parasites inflict on human’s remains a major issue worldwide. Accurate diagnosis of a parasite is the first step to fight against these parasitism and infectious diseases. To detect, identify and determine the prevalence of parasites among patients with abdominal symptoms in and around village banger, Dewas and also to compare different methods of wet mount preparations for stool examination. Stool samples from a total of 200 patients were collected and subjected for routine macroscopic as well as microscopic examination of stool by five wet mount methods namely saline, iodine, iodine-glycerol, KOH and LPCB. Concentration methods (saturated salt flotation and formal ether sedimentation) were also performed for sample negative for direct wet mount. Overall parasitic infestation was detected in 34.5% patients. This study revealed mixed parasite infestations in 7 cases. By direct wet mount, positivity was 46, which is increased to 73 after concentration of stool sample. The prevalence of protozoan infestations was much higher than the prevalence of helminth infestations and LPCB detected all parasitic forms in higher number. This study revealed a noticeable evidence of parasitisation which demands up gradation of sanitation and better living standards. Thus a approach for the control of parasitic infestation, based on streamlined health education and improved sanitation standards, is necessary measure. The use of Lacto phenol cotton blue stain along with saline wet mounts for routine microscopic examination of stools for ova& cyst can be justified by this study.


Keywords: LPCB, Iodine- Glycerol, Lugol’s iodine, KOH, Stool, parasites
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How to cite this article:

Khyati Jain, Arjun S. Gurjar and Madhurendra S. Rajput. 2018. A Cross Sectional Study for Prevalence of Intestinal Parasitic Infestation by Using Saline, Iodine, Glycerol-Iodine, KOH and LPCB Wet Mount Preparations of Stool Samples From Patients Attending Aims, Dewas.Int.J.Curr.Microbiol.App.Sci. 7(5): 3449-3457. doi: https://doi.org/10.20546/ijcmas.2018.705.399