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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(5): 3240-3250
DOI: https://doi.org/10.20546/ijcmas.2018.705.379


Identification and Functional Characterization an Ortholog of OsENOD93-1 Gene in Wheat Using in-silico Approach
Anuj Kumar1, 2, Avneesh Kumar3, Pankaj Tyagi4* and Krishna Pal Singh1,5
1Advanced Centre for Computational and Applied Biotechnology, Uttarakhand Council for Biotechnology, Dehradun, Uttarakhand-248007, India
2Dr. A.P.J. Abdul Kalam Technical University, Lucknow, Uttar Pradesh-226031, India
3Department of Botany, Akal University, Talwandi Sabo, Bathinda, Punjab-151302, India
4Department of Biotechnology, Meerut Institute of Engineering and Technology, Meerut, Uttar Pradesh-250005, India
5Department of Molecular Biology, Biotechnology and Bioinformatics, College of Basic Sciences and Humanities, CCS Haryana Agricultural University, Hisar, Haryana-125004, India
*Corresponding author
Abstract:

Nitrogen is an essential macromolecule necessary for suitable growth and development of plants by playing a vital role in biochemical and physiological functions throughout the life cycle. Early node 93 (ENOD93-1) gene is well known for significantly responding to both N induction and N reduction in plants, but little is known about the structure and function of the ENOD93-1 protein in cereal crops. In this study, a putative ortholog of rice OsENOD93-1 gene was identified in the wheat genome. BLASTP analysis showed the OsENOD93-1 shared the 80% similarity with wheat TRIAE_CS42_4AL_TGACv1_ 288261_AA0942630.1 scaffold. Present study describes the location of TaENOD93-1 gene on4AL chromosome of wheat. MicroRNA target analysis revealed that TaENOD93-1 gene targeting by miR6201. Phylogeny tree suggested that identified putative ortholog shared the evolutionary relationship with monocots. The three dimensional structure of TaENOD93-1 protein was predicted using computer-aided molecular modeling technique followed by structure evaluation using Ramachandran plot. The primary goal of this study was to mapping the chromosomal location and functional annotation of TaENOD93-1 gene in wheat using bioinformatics approach. The identification of putative ortholog of OsENOD93-1 gene in wheat has opened up new opportunities to improve the nitrogen use efficiency (NUE) in wheat through molecular breeding approach.


Keywords: Nitrogen, Early node, In-silico, Chromosomal mapping, Functional annotation, Homology modeling
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How to cite this article:

Anuj Kumar, Avneesh Kumar, Pankaj Tyagi and Krishna Pal Singh. 2018. Identification and Functional Characterization an Ortholog of OsENOD93-1 Gene in Wheat Using in-silico Approach.Int.J.Curr.Microbiol.App.Sci. 7(5): 3240-3250. doi: https://doi.org/10.20546/ijcmas.2018.705.379