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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(5): 3206-3218
DOI: https://doi.org/10.20546/ijcmas.2018.705.375


Isolation, Molecular and Electron Microscopic Detection of PPR Virus from Suspected Outbreaks of PPR in Goats of North – Eastern Karnataka
K.C. Mallinath1, A. Basavaraj1, Amitha R. Gomes2, B.H. Jagadish3, M. Shivaraj2, R. Bhoyar4, S.M. Byregowda2, N.A. Patil4, C. Jagannathrao4, P. Ubhale1 and Phani Kashyap3
1Department of Veterinary Microbiology, Veterinary College, Bidar-585401, Karnataka, India
2IAH &VB- Bengaluru, Karnataka, India
3ICAR-NIVEDI- Bengaluru, Karnataka, India
4Veterinary College, Bidar, Karnataka, India
*Corresponding author
Abstract:

Region wise monitoring of the PPR virus by active surveillance is essential as it aids in strategic planning of measures of disease control and prevention. The North Eastern Karnataka with sizable sheep and goat population experiences the outbreaks of PPR but the attempts to isolate and characterize the prevailing PPR virus is limited. Hence, the present research work was carried out with the objective of isolation and characterization of PPR virus isolates circulating in North Eastern Karnataka by microscopic (TEM) and molecular (RT-PCR) methods. A total of 15 suspected PPR outbreaks were attended and different types of clinical samples like nasal, oral, ocular, rectal swabs and blood from ailing kids and goats were collected. The samples were initially screened for the presence of PPR virus by observing the infected Vero-cells for development of characteristic CPE. The isolates showing CPE were further confirmed for the virus by RT-PCR using N and F genes of PPR virus. The PCR confirmed isolates were subjected to transmission electron microscopy (TEM). A total of six PPR isolates were obtained representing five districts of North Eastern Karnataka which were confirmed by RT-PCR. The TEM images showed pleomorphic virion particles with varying sizes indicative of Morbilli-virus. These findings provide the first documentation of PPR presence by microscopic and molecular characterization in the region.


Keywords: PPR, TEM, Morbilli-virus, N, F gene etc.
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How to cite this article:

Mallinath, K.C., A. Basavaraj, Amitha R. Gomes, B.H. Jagadish, M. Shivaraj, R. Bhoyar, S.M. Byregowda, N.A. Patil, C. Jagannathrao, P. Ubhale and Phani Kashyap. 2018. Isolation, Molecular and Electron Microscopic Detection of PPR Virus from Suspected Outbreaks of PPR in Goats of North – Eastern Karnataka.Int.J.Curr.Microbiol.App.Sci. 7(5): 3206-3218. doi: https://doi.org/10.20546/ijcmas.2018.705.375