National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Genetic variability among the 24 isolates of S. rolfsii was studied by using molecular markers like ITS-PCR and RAPD primers. Amplification of ITS region of rDNA with specific ITS1 and ITS4 universal primers produced approximately 650 to 700 bp in all the isolates of the fungus confirmed that all the isolates obtained are Sclerotium rolfsii and were sequenced. Identity of the isolates was confirmed with sequences of NCBI data base of S. rolfsii. Among the twenty four isolates, four random primers viz., UBC-467, UBC-482, UBC-485 and UBC-489 generated reproducible polymorphism. Amplified products with all the primers have showed polymorphic and distinguishable banding pattern indicating the genetic diversity among all the isolates of S. rolfsii. A total of 342 reproducible and scorable polymorphic bands ranging approximately as low as 150 bp to as high as 2000 bp was generated with four primers among the twenty four isolates studied. All the twenty four isolates were grouped into three main clusters indicating there is genetic diversity in the isolates of S. rolfsii. Cluster I contained thirteen isolates, main cluster divided into two sub clusters, sub cluster I had eight isolates (Sr21, Sr20, Sr19, Sr18, Sr22, Sr24, Sr17 and Sr23) and subcluster II had five isolates (Sr5, Sr2, Sr1, Sr6 and Sr4). Main cluster II has two sub clusters, sub cluster I consisted of six isolates (Sr8, Sr3, Sr7, Sr12, Sr15 and Sr14) and in sub cluster II three isolates (Sr16, Sr10 and Sr13) were grouped. Cluster III consisted of two isolates (Sr9 and Sr11).
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