National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present investigation entitled “Optimization Protocol of DNA isolation and PCR in Muskmelon (Cucumis melo L.) by RAPD Marker” was carried out at Department of Plant Biotechnology SDMVM’s College of Agricultural Biotechnology, Georai Tanda, Paithan Road, Aurangabad (M.S.), 431001. Six different commercial verities of muskmelon plant were studied for this research. Standardization protocol of DNA isolation from muskmelon - One method (CTAB) was studied for obtaining good quality DNA from muskmelon. It is showing that when C-TAB method were used good quality of DNA obtained and its quantity 100ng. When C-TAB method used for isolation of DNA from Muskmelon, three extraction buffers were used for optimizing condition for DNA isolation from C-TAB method. Five primers were used for testing polymorphism and monomorphism percentage. Out of five primer A14 primer showed 22.22% polymorphism and remaining primer showed monomorphism. Total primer showed 4.1% polymorphism and 95.10% monomorphism.
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