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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(5): 2678-2684
DOI: https://doi.org/10.20546/ijcmas.2018.705.310


Optimization Protocol of DNA Isolation and PCR in Muskmelon (Cucumis melo L.) by RAPD Marker
Jotshana Manik Maske*, Rajput Charansing Amarsing and Zote Rahul Keshavrao
Department of Plant Biotechnology SDMVM’s College of Agricultural Biotechnology, Georai Tanda, Paithan Road, Aurangabad (M.S.)-431001, India
*Corresponding author
Abstract:

The present investigation entitled “Optimization Protocol of DNA isolation and PCR in Muskmelon (Cucumis melo L.) by RAPD Marker” was carried out at Department of Plant Biotechnology SDMVM’s College of Agricultural Biotechnology, Georai Tanda, Paithan Road, Aurangabad (M.S.), 431001. Six different commercial verities of muskmelon plant were studied for this research. Standardization protocol of DNA isolation from muskmelon - One method (CTAB) was studied for obtaining good quality DNA from muskmelon. It is showing that when C-TAB method were used good quality of DNA obtained and its quantity 100ng. When C-TAB method used for isolation of DNA from Muskmelon, three extraction buffers were used for optimizing condition for DNA isolation from C-TAB method. Five primers were used for testing polymorphism and monomorphism percentage. Out of five primer A14 primer showed 22.22% polymorphism and remaining primer showed monomorphism. Total primer showed 4.1% polymorphism and 95.10% monomorphism.


Keywords: DNA, PCR, Muskmelon, RAPD
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How to cite this article:

Jotshana Manik Maske, Rajput Charansing Amarsing and Zote Rahul Keshavrao. 2018. Optimazation Protocol of DNA Isolation and PCR in Muskmelon (Cucumis melo L.) by RAPD MarkerInt.J.Curr.Microbiol.App.Sci. 7(5): 2678-2684. doi: https://doi.org/10.20546/ijcmas.2018.705.310