National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Gram negative enteric bacilli are a leading cause for sepsis in children and adults. The infections caused by Enterobacteriaceae are community and hospital-acquired. These bacteria have the potential to spread in the hospital environment and also across continents. Current study was designed to characterise Escherichia coli isolates from blood stream infection and identify the genes responsible for carbapenemase production and correlate the results with MHT. Ten consecutive isolates of E. coli resistant to imipenem (10 µg)/meropenem (10 µg) isolated from individuals with blood stream infection were selected for testing. Among the 10 isolates tested, 4 were positive with MHT. Among these, 3 of them harboured the genes for carbapenemase production as detected by PCR. This included 1 NDM, 1 IMP and 1 OXA-48 genes. Three of the isolates which tested negative with MHT were found to be positive for the NDM gene by PCR thus giving a false negative result with MHT. However, a total of 2 isolates were negative for the genes mentioned above thus giving a false positive result with MHT. Three isolates gave a negative result with MHT among which 2 of them did not have any of the carbapenemase genes by PCR. A total of 4 out of 10 isolates gave a positive result with PCR for the genes tested. The most common gene conferring resistance to carbapenems for E. coli in the study was NDM (3 out of 10 isolates). We found one isolate each positive for IMP and OXA-48 genes. We conclude that accurate and timely identification of genes conferring resistance to carbapenems serves as an important tool in instituting therapy and infection control measures. Conventional multiplex PCR is a sensitive technique to detect multiple genes.
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