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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(5): 2548-2555
DOI: https://doi.org/10.20546/ijcmas.2018.705.293


Comparison of Different Methods for Detection of Biofilm Formation in Staphylococcus aureus in a Tertiary Care Hospital
Prasanna Gupta1, Manish Kumar Diwakar2*, Santosh Verma3 and Neha Srivastava4
1Department of Microbiology, NIMS Medical college, Jaipur, Rajasthan, India
2Department of Microbiology, 3Department of Community Medicine, MLB Medical College, Jhansi (UP), India
4Department of Microbiology, Rama Medical College, Kanpur (UP), India

*Corresponding author
Abstract:

Biofilm is the population of bacterial cells growing on the biotic and abiotic surfaces embedded in a matrix of extracellular polysaccharide, which facilitate the adherence of microorganism. This study is aimed to compare and evaluate different Methods for detection of Biofilm formation in Staphylococcus aureus isolates. For comparison of different methods of Biofilm production, 100 isolates of Staphylococcus aureus (50 MRSA and 50 MSSA) were taken and screened by tissue culture plate method, tube method in different condition and Congo Red Agar (CRA) plate method. Out of 100 isolates of Staphylococcus aureus, 19% shows Strong biofilm formation, while 33 % showed moderate and 48 % isolates showed weak/No biofilm production by TCP Methods. In TM-Gr-1: 10%, 28% and 62%; in TM-Gr-2: 10%, 20% and 70%; in TM-Gr-3: 16, 29% and 55% isolates showed strong, moderate and weak/ No biofilm production respectively. The CRA Method showed 17% biofilm production. The TCP method was found to be most sensitive screening method for detection of biofilm formation by staphylococcus aureu. MRSA isolates were more Biofilm producers than MSSA.


Keywords: Biofilm, Tissue culture plate method, Congo red agar, Staphylococcus aureus, MRSA
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How to cite this article:

Prasanna Gupta, Manish Kumar Diwakar, Santosh Verma and Neha Srivastava. 2018. Comparison of Different Methods for Detection of Biofilm Formation in Staphylococcus aureus in a Tertiary Care Hospital.Int.J.Curr.Microbiol.App.Sci. 7(5): 2548-2555. doi: https://doi.org/10.20546/ijcmas.2018.705.293