National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In this study, oocytes were recovered from 200 numbers of goats’ ovaries from different slaughterhouses for subsequent use in-vitro maturation, enucleation, reconstruction and embryo culture to produce cloned embryos. The collected oocytes were classified into three categories (Grade A, B and C) based on their appearance of homogenous granular ooplasm and layers of cumulus cells surrounding oocytes under a stereozoom microscope. The average oocyte recovery, In-vitro maturation and the enucleation rates were found to be 3.36 per ovary, 60.37 per cent and 75.52 per cent respectively. Two enucleated (demi) oocytes were fused to one fibroblast cells to produce reconstructed oocyte and the average success rate was recorded as 23.95 per cent. When the reconstructed oocytes were parthenogenetically activated, followed by in-vitro culture of embryos in mSOF media, the average clone embryo production was 6.38 per cent. Grade A oocytes yielded higher maturation rate and subsequent embryo production in-vitro.
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