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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(5): 2518-2529
DOI: https://doi.org/10.20546/ijcmas.2018.705.290


Handmade Cloning for Embryo Production in Goat, Assam, India
Monika Borah1, Nikhil C. Nath2, Probodh Borah1, Biju Borah3*, Girin Hazarika1 and Padma L. Borah4
1Department of Animal Biotechnology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam, India
2Department of Veterinary Physiology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam, India
3ICAR-NRC on Yak, Dirang, Arunachal Pradesh, India
4Molbio Diagnostics Pvt. Ltd, Assam, India
*Corresponding author
Abstract:

In this study, oocytes were recovered from 200 numbers of goats’ ovaries from different slaughterhouses for subsequent use in-vitro maturation, enucleation, reconstruction and embryo culture to produce cloned embryos. The collected oocytes were classified into three categories (Grade A, B and C) based on their appearance of homogenous granular ooplasm and layers of cumulus cells surrounding oocytes under a stereozoom microscope. The average oocyte recovery, In-vitro maturation and the enucleation rates were found to be 3.36 per ovary, 60.37 per cent and 75.52 per cent respectively. Two enucleated (demi) oocytes were fused to one fibroblast cells to produce reconstructed oocyte and the average success rate was recorded as 23.95 per cent. When the reconstructed oocytes were parthenogenetically activated, followed by in-vitro culture of embryos in mSOF media, the average clone embryo production was 6.38 per cent. Grade A oocytes yielded higher maturation rate and subsequent embryo production in-vitro.


Keywords: Cloning, Embryo, Goat, Handmade, Production
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How to cite this article:

Monika Borah, Nikhil C. Nath, Probodh Borah, Biju Borah, Girin Hazarika and Padma L. Borah. 2018. Handmade Cloning for Embryo Production in Goat, Assam, India.Int.J.Curr.Microbiol.App.Sci. 7(5): 2518-2529. doi: https://doi.org/10.20546/ijcmas.2018.705.290