National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Cancer is a major public health burden in both developed and developing countries. Plant derived agents are being used for the treatment of cancer. Plants such as herbs have been used in folk medicine for centuries in most of the cultures throughout the world. The North Eastern regions of India are considered to be one of the biodiversity hotspots. Paris polyphylla (Paris polyphylla Smith. in Rees. Cyclop.26: paris n.2.1813; Hook.f., Fl. Britis India 6:362.1892., Liliaceae) is one of the indigenous medicinal plants of Manipur that have been used as medicine for over many years. The ground dried rhizomes of Paris polyphylla increases the surface area which enhance the rate of extraction using ethanol and petroleum ether for Soxhlet extraction techniques. The antioxidant activity by DPPH (1,1-diphenyl-2picrylhydrazyl) assay showed 68μg/ml of the ethanol extract and the total phenol content by Folin’s Ciocalteu reagent showed the presence of antioxidant property with a total phenol concentration of 0.68mg/g catechol and 0.47mg/g catechol with the ethanol and petroleum ether extracts respectively. Regarding the valuable usage of Paris polyphylla, the tissue culture from the rhizome explant was conducted on Murashige and Skoog (MS) media supplemented with 20.0 g/l sucrose, 8.0 g/l agar with different concentrations of 6benzylaminopurine (BAP), a-naphtaleneacetic acid (NAA) and Kinetin (KIN) to find a standard protocol for its mass production. The medium MS + 2mg/l BAP + 2mg/l NAA + 2.5 g/l Activated charcoal gave the best result of shoot regeneration. The findings also pointed to the possibility of consistent mass production of reliable planting materials like medicinal herbs for large scale in vitro micropropagation that may greatly help in pharmaceutical companies.
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