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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(5): 2355-2367
DOI: https://doi.org/10.20546/ijcmas.2018.705.271


Antioxidant Activity and Polyphenol Contents of Paris polyphylla Smith and Prospects of in situ Conservation
Warepam Jesmi Devi1*, J.M. Laishram1 and Supriyo Chakraborty2
1College of Agriculture, Central Agricultural University, India
2Assam University Silchar, India
*Corresponding author
Abstract:

Cancer is a major public health burden in both developed and developing countries. Plant derived agents are being used for the treatment of cancer. Plants such as herbs have been used in folk medicine for centuries in most of the cultures throughout the world. The North Eastern regions of India are considered to be one of the biodiversity hotspots. Paris polyphylla (Paris polyphylla Smith. in Rees. Cyclop.26: paris n.2.1813; Hook.f., Fl. Britis India 6:362.1892., Liliaceae) is one of the indigenous medicinal plants of Manipur that have been used as medicine for over many years. The ground dried rhizomes of Paris polyphylla increases the surface area which enhance the rate of extraction using ethanol and petroleum ether for Soxhlet extraction techniques. The antioxidant activity by DPPH (1,1-diphenyl-2picrylhydrazyl) assay showed 68μg/ml of the ethanol extract and the total phenol content by Folin’s Ciocalteu reagent showed the presence of antioxidant property with a total phenol concentration of 0.68mg/g catechol and 0.47mg/g catechol with the ethanol and petroleum ether extracts respectively. Regarding the valuable usage of Paris polyphylla, the tissue culture from the rhizome explant was conducted on Murashige and Skoog (MS) media supplemented with 20.0 g/l sucrose, 8.0 g/l agar with different concentrations of 6benzylaminopurine (BAP), a-naphtaleneacetic acid (NAA) and Kinetin (KIN) to find a standard protocol for its mass production. The medium MS + 2mg/l BAP + 2mg/l NAA + 2.5 g/l Activated charcoal gave the best result of shoot regeneration. The findings also pointed to the possibility of consistent mass production of reliable planting materials like medicinal herbs for large scale in vitro micropropagation that may greatly help in pharmaceutical companies.


Keywords: Paris polyphylla, Cancer, Antioxidant, Micropropagation
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How to cite this article:

Warepam Jesmi Devi, J.M. Laishram and Supriyo Chakraborty. 2018. Antioxidant Activity and Polyphenol Contents of Paris polyphylla Smith and Prospects of in situ Conservation.Int.J.Curr.Microbiol.App.Sci. 7(5): 2355-2367. doi: https://doi.org/10.20546/ijcmas.2018.705.271