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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(5): 1991-2001
DOI: https://doi.org/10.20546/ijcmas.2018.705.234


Cloning, Sequencing and In Silico Characterization of OmpF Protein of Salmonella typhimurium for its Immune-Potential
Nimisha Soman1, Prejit1, 2*, P.T. Pratheesh1, S.H. Mahesh1 and V.K. Vinod1, 2
1Department of Veterinary Public Health, CV&AS, Kerala Veterinary and Animal Sciences University, Pookode, Wayanad, Kerala - 673576, India
2Centre for One Health Education, Advocacy, Research and Training, Kerala Veterinary and Animal Sciences University, Pookode, Wayanad, Kerala - 673576, India
*Corresponding author
Abstract:

Salmonella is a major foodborne bacterial pathogen responsible for millions of cases of infection and thousands of death in humans across the globe annually. Poultry and poultry products are considered to be the major source of Salmonella infection in humans. The economic loss caused by salmonellosis is very huge in case of poultry industry. Vaccination of the poultry is found to efficiently reduce the zoonotic risks associated with Salmonella infection. Virulence nature of outer membrane proteins of Gram-negative bacteria Salmonella is found to efficiently generate immunity in host system. Hence, several efforts have been made to develop an outer membrane protein based vaccine and were found to be successful. So, in the present study outer membrane protein F (OmpF) of Salmonella typhimurium was cloned, sequenced and in silico analysis was carried out to study its structural and immunological characteristics. Antigenic indexing, Epitope mapping and MHC mapping were performed using various prediction servers and online tools. Presence of eight major B cell epitopes indicated ability of OmpF to elicit humoral immunity. VaxiJen score of 0.85 further confirmed the antigenic nature of the epitope. T-cell epitope mapping identified several epitopes with high MHC binding affinity representing ability to provoke cell mediated immunity. The results from the study indicated that OmpF protein of Salmonella typhimurium is a strong candidate for developing recombinant vaccine against salmonellosis in poultry.


Keywords: Salmonella typhimurium, OmpF, In silico analysis, Vaccine
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How to cite this article:

Nimisha Soman, Prejit, P.T. Pratheesh, S.H. Mahesh and Vinod, V.K. 2018. Cloning, Sequencing and In Silico Characterization of OmpF Protein of Salmonella typhimurium for its Immune-Potential.Int.J.Curr.Microbiol.App.Sci. 7(5): 1991-2001. doi: https://doi.org/10.20546/ijcmas.2018.705.234